Skip to content
1887

Microbiology Society logo Microbiology

Volume 71, Issue 3

The Effect of Competence Regime on Competence, DNA Absorption and Integration of DNA in Cultures of Bacillus subtilis Free

Abstract

Summary: In cultures of subjected to different competence regimes, the size of the competent fraction, the amount of DNA absorbed per competent cell and the efficiency of integration have been measured. Supplementation of cultures with competence enhancing amino acids results in a fourfold increase in the frequency of transformation compared to supplementation with casein hydrolysate. The increased transformability in the presence of the competence enhancing amino acids is attributed to a twofold increase in the size of the competent fraction in addition to a twofold increase in the amount of DNA absorbed per competent cell. The efficiency of integration (ratio of the probability of transformation per competent cell and the chromosome equivalent of DNA absorbed per competent cell) is approximately 0·4 in both conditions. On the basis of single strand integration this value is consistent with the finding that in both competence regimes approximately 20 % of initially absorbed [H]thymidine-labelled donor DNA is finally retained during subsequent incubation.

  • Accepted:
  • Published Online:
© Society for Gerenal Microbiology 1972
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-71-3-415
1972-08-01
2025-03-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/71/3/mic-71-3-415.html?itemId=/content/journal/micro/10.1099/00221287-71-3-415&mimeType=html&fmt=ahah

References

  1. BODMER W. F. 1965; Recombination and integration in Bacillus subtilis transformation: involvement of DNA synthesis.. Journal of Molecular Biology 14:534–557
     [Google Scholar]
  2. BRAY G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter.. Annals of Biochemistry 1:279–285
     [Google Scholar]
  3. BRESLER S. E., MOSEVITSKII M. I., TIMKOVSKII A. L. 1963; The irregular penetration of DNA into cells, accompanying bacterial transformation.. Doklady Akademii Nauk SSSR 149:721–724
     [Google Scholar]
  4. BURTON K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.. Biochemical Journal 62:315–323
     [Google Scholar]
  5. CAHN F. H., Fox M. S. 1968; Fractionation of transformable bacteria from competent cultures of Bacillus subtilis on renografin gradients.. Journal of Bacteriology 95:867–875
     [Google Scholar]
  6. DUBNAU D., DAVIDOFF-ABELSON R. 1971; Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex.. Journal of Molecular Biology 56:209–221
     [Google Scholar]
  7. EBERLE H., LARK K. G. 1967; Chromosome replication in Bacillus subtilis cultures growing at different rates.. Proceedings of the National Academy of Sciences of the United States 57:95–101
     [Google Scholar]
  8. Goodgal S. H., Herriott R. M. 1961; Studies on transformation of Hemophilus influenzae. I. Competence.. Journal of General Physiology 44:1201–1227
     [Google Scholar]
  9. KAMMEN H. O., BELOFF R. H., CANELLAKIS E. S. 1966; Transformation in Bacillus subtilis. I. Role of amino acids in stabilization of transformants.. Biochimica et biophysica acta 123:39–55
     [Google Scholar]
  10. ROSENTHAL P. N., Fox M. S. 1970; Effects of desintegration of incorporated 3H and 32P on the physical and biological properties of DNA.. Journal of Molecular Biology 54:441–463
     [Google Scholar]
  11. SIEGEL S. 1956 Non parametric Statistics,. pp. 116–127 New York: McGraw-Hill Inc. Tokyo:: Kogakusha Company.;
     [Google Scholar]
  12. SOMMA S., POLSINELLI M. 1970; Quantitative autoradiographic study of competence and deoxyribonucleic acid incorporation in Bacillus subtilis.. Journal of Bacteriology 101:851–855
     [Google Scholar]
  13. SPIZIZEN J. 1958; Transformation of biochemically deficient strains of Bacillus subtillis by deoxyribonu-cleate.. Proceedings of the National Academy of Sciences of the United States 44:1072–1078
     [Google Scholar]
  14. SUEOKA N., YOSHIKAWA H. 1963; Regulation of chromosome replication in Bacillus subtilis.. Cold Spring Harbor Symposia on Quantitative Biology 28:47–54
     [Google Scholar]
  15. VENEMA G., PRITCHARD R. H., VENEMA-SCHRODER T. 1965; Fate of transforming deoxyribonucleic acid in Bacillus subtilis.. Journal of Bacteriology 89:1250–1255
     [Google Scholar]
  16. Vermeulen C. A., Venema G. 1971; Autoradiographic estimation of competence and the relationship between competence and transformability in cultures of Bacillus subtilis.. Journal of General Microbiology 69:239–252
     [Google Scholar]
  17. Vestri R., Felicetti L., Lostia O. 1966; Transformation by hybrid DNA in Bacillus subtilis.. Nature London: 209:1154–1155
     [Google Scholar]
  18. WILSON G. A., BOTT K. F. 1968; Nutritional factors influencing the development of competence in the Bacillus subtilis transformation system.. Journal of Bacteriology 95:1439–1449
     [Google Scholar]
  19. YOSHIKAWA H., O’SULLIVAN A., SUEOKA N. 1964; Sequential replication of the Bacillus subtilis chromosome. III. Regulation of initiation.. Proceedings of the National Academy of Sciences of the United States 52:973–980
     [Google Scholar]
  20. YOSHIKAWA H., SUEOKA N. 1963; Sequential replication of Bacillus subtilis chromosome. I. Comparison of marker frequencies in exponential and stationary growth phases.. Proceedings of the National Academy of Sciences of the United States 49:559–566
     [Google Scholar]
  21. YOUNG F. E., SPIZIZEN J., CRAWFORD T. P. 1963; Biochemical aspects of competence in the Bacillus subtilis transformation system. I. Chemical composition of cell walls.. Journal of Biological Chemistry 238:3119–3125
     [Google Scholar]
/content/journal/micro/10.1099/00221287-71-3-415
Loading
The Effect of Competence Regime on Competence, DNA Absorption and Integration of DNA in Cultures of Bacillus subtilis
Microbiology 71, 415 (1972); https://doi.org/10.1099/00221287-71-3-415
/content/journal/micro/10.1099/00221287-71-3-415
/content/journal/micro/10.1099/00221287-71-3-415
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error