1887

Abstract

Summary: In cultures of subjected to different competence regimes, the size of the competent fraction, the amount of DNA absorbed per competent cell and the efficiency of integration have been measured. Supplementation of cultures with competence enhancing amino acids results in a fourfold increase in the frequency of transformation compared to supplementation with casein hydrolysate. The increased transformability in the presence of the competence enhancing amino acids is attributed to a twofold increase in the size of the competent fraction in addition to a twofold increase in the amount of DNA absorbed per competent cell. The efficiency of integration (ratio of the probability of transformation per competent cell and the chromosome equivalent of DNA absorbed per competent cell) is approximately 0·4 in both conditions. On the basis of single strand integration this value is consistent with the finding that in both competence regimes approximately 20 % of initially absorbed [H]thymidine-labelled donor DNA is finally retained during subsequent incubation.

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/content/journal/micro/10.1099/00221287-71-3-415
1972-08-01
2022-07-04
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