Transformation in Free

Abstract

Summary: Genetic recombination occurs with a rather high frequency (up to 10) in mixed cultures of auxotrophic and streptomycin-resistant mutant derivatives of strain 9 growing on agar medium. It is shown that transformation by DNA is responsible for this recombination. Certain other newly isolated strains resemble 9 in being competent for transformation and are interfertile with each other and with 9. Some other strains are incompetent but can act as donors to the first set of strains. A fully synthetic minimal medium and conditions for efficient mutagenesis have been defined.

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1972-07-01
2024-03-29
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