Summary: Thin sections, freeze-etch preparations and carbon replicas of Thermoactinomyces vulgaris sporangia and endospores were examined in the electron microscope. The outer spore coat contained parallel arrays of long fibrous striations spaced at about 5 nm; there appeared to be several layers with a different orientation of the fibres in each. The outer coat was polyhedral with slightly concave pentagonal and hexagonal faces. A combination of geometrical considerations, analysis of the patterns of faces seen in the replicas and freeze-etchings, and model building suggested that the polyhedron had 12 pentagonal and approximately 12 hexagonal faces.
BRADLEYD. E.,
WILLIAMSD. J.1957; An electron microscope study of the spores of some species of the
genus Bacillus using carbon replicas.. Journal of General MicrobioZogy 17:75–79
CROSST.,
JOHNSTOND. W.1971Thermoactinomyces vulgaris. 11. Distribution in natural habitats.. In Spore Research1971 pp 315–330 Edited by
BarkerA. N.,
GouldG. W.,
WolfJ.
London: Academic Press;
DOROKHOVAI. A.,
AGREN. S.,
KALAKUTSKIIL. V.,
KRASSILNIKOVN. A.1968; Fine structure of spores in a thermophilic actinomycete, Micromonospora vulgaris.. Journal of General and Applied Microbiology 14:295–303
DOROKHOVAL. A.,
AGREN. S.,
KALAKUTSKIIL. V.,
KRASSILNIKOVN. A.1970; Electron microscopic study of spore formation in Micromonospora vulgaris.. Microbiology (a translation of Mikrobiologiya) 39:589–593
GOULDG. W.,
STUBBSJ. M.,
KINGW. L.1970; Structure and composition of resistant layers in bacterial spore coats.. Journal of General Microbiology 60:347–355
HOPWOODD. A.,
FERGUSONH. M.1970; Genetic recombination in a thermophilic actinomycete, Thermoactinornyces vulgaris.. Journal of General Microbiology 63:133–136
WILLIAMSS. T.,
DAVIESF. L.1967; Use of a scanning electron microscope for the examination of actinomycetes.. Journal of General Microbiology 48:171–177