1887

Abstract

Summary: DNA synthesis in and strains of is strongly inhibited without delay by low doses of gamma-radiation. The synthesis that does occur in these strains appears to take place mainly on chromosomes that have not received a lethal hit. Low doses produce much less inhibition of DNA synthesis in and wild-type strains. We conclude that the gene product acts immediately, certainly before DNA replication, in the repair of lesions produced by gamma-irradiation. In bacteria the gene product is not required for continued DNA synthesis, although the synthesis which occurs in strains may be abnormal since it is not reflected in increased survival. The gene product is not necessary for continued DNA replication, suggesting that its time of action in repair may be at a later stage.

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/content/journal/micro/10.1099/00221287-71-2-359
1972-07-01
2024-04-25
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References

  1. BREITMAN T.R., BRADFORD R.M., CANNON W.D. 1967; Use of exogenous deoxythymidylic acid to label the deoxyribonucleic acid of growing wild-type Escherichia coli.. Journal of Bacteriology 93:1471–1472
    [Google Scholar]
  2. BUCHWALD M., BRITTEN R.J. 1963; Incorporation of ribonucleic acid bases into the metabolic pool and RNA of E. coli.. Biophysical Journal 3:155–166
    [Google Scholar]
  3. CAIRNS J., DAVERN C.I. 1966; Effect of 32P decay upon DNA synthesis by a radiation-sensitive strain of Escherichia coli.. Journal of Molecular Biology 17:418–427
    [Google Scholar]
  4. DONCH J.J., GREEN M.H.L., GREENBERG J. 1968; Interaction of the exr and Zon genes in Escherichia coli.. Journal of Bacteriology 96:1704–1710
    [Google Scholar]
  5. GRAY W.J.H., MIDGLEY J.E.M. 1971; The control of ribonucleic acid synthesis in bacteria: The synthesis and stability of ribonucleic acid in rifampicin-inhibited cultures of Escherichia coli.. Biochemical Journal 122:161–169
    [Google Scholar]
  6. GREEN M. H. L., BRIDGES B.A., RIAZUDDIN S. 1971; Effect of gamma-radiation on the donor ability of recA and recA+ strains of Escherichia coli.. Journal of General Microbiology 67:63–68
    [Google Scholar]
  7. GREEN M.H.L., GRAY W.J.H., MURDEN D.J., BRIDGES B.A. 1971; Influence of growth phase on U.V. induced lethality and DNA breakdown in a Kornberg polymerase deficient resA strain of Escherichia coli.. Molecular and General Genetics 112:110–116
    [Google Scholar]
  8. HOWARD-FLANDERS P., BOYCE R.P. 1966; DNA repair and genetic recombination: Studies on mutants of Escherichia coli defective in these processes. Radiation Research Supplement 6:156–184
    [Google Scholar]
  9. KAPP D.J., SMITH K.C. 1970; Repair of radiation-induced damage in Escherichia coli. II. Effect of rec and uvr mutations on radiosensitivity and repair of X-ray induced single strand breaks in deoxyribonucleic acid.. Journal of Bacteriology 103:49–54
    [Google Scholar]
  10. MCGRATH R.A., WILLIAMS R.W. 1966 Reconstruction in vivo of irradiated Escherichia coli deoxy-ribonucleic acid; the rejoining of broken piece.. Nature London; 212534–535
    [Google Scholar]
  11. MORIMYO M., HORII Z.I., SUZUKI K. 1968; Appearance of low molecular weight DNA in a Rec- mutant of Escherichia coli K-12 irradiated with X-rays. . Journal of Radiation Research 9:19–25
    [Google Scholar]
  12. SMITH K.C. 1971 The roles of genetic recombination and DNA polymerase in the repair of damaged DNA.. In Photophysiology 6 Edited by Giese A.C. New york:: Academic Press;
    [Google Scholar]
  13. TOWN C.D., SMITH K.C., KAPLAN H.S. 1971 DNA polymerase required for the rapid rejoining of X-ray induced DNA strand breaks in vivo.. Science, New York: 172851–853
    [Google Scholar]
  14. WEHRLI W., KNUSEL F., SCHMID K., STAEHELIN M. 1968; Interaction of rifamycin with bacterial RNA polymerase.. Proceedings of the National Academy of Sciences of the United States of America 61:667–673
    [Google Scholar]
  15. WILLETS N.S., CLARK A.J. 1969; Characteristics of some multiple recombination-deficient strains of Escherichia coli.. Journal of Bacteriology 100:231–239
    [Google Scholar]
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