Summary: Macerates of buds of apple, cottonwood and white pine were cultured for bacteria, fungi and yeasts periodically during the spring. Fewer than 1000 propagules of each were found in most buds. As the growing season progressed, the surface of parts of buds of herbaceous plants and grape was examined microscopically for micro-organisms. Fungi and yeasts were rare. Numbers of bacteria were seen on > 60% of the buds of red clover, soybean, cucumber, turnip and grape. Macerated parts of soybean plants from the field and greenhouse were assayed for bacteria. Large numbers of bacteria were associated with buds, flowers and small pods from field plants, and comparatively few were associated with mature leaves. In contrast, few bacteria were associated with any of these organs from greenhouse plants.


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