%0 Journal Article %A CLARKE, PATRICIA H. %T Biochemical and Immunological Comparison of Aliphatic Amidases Produced by Pseudomonas Species %D 1972 %J Microbiology, %V 71 %N 2 %P 241-257 %@ 1465-2080 %R https://doi.org/10.1099/00221287-71-2-241 %I Microbiology Society, %X Summary: Strains of Pseudomonas aeruginosa, P. putida, P. acidovorans and P. cepacia were tested for growth on minimal salt media containing aliphatic amides. All strains of P. aeruginosa, P. acidovorans, P. cepacia and some strains of P. putida biotype A grew on acetamide; some strains of P. putida and P. acidovorans also grew on butyramide. The inducible aliphatic amidases had both hydrolase and transferase activities and were similar in substrate specificities. All species hydrolysed propionamide more rapidly than acetamide while acetamide was the best substrate for the transferase reaction. The rate of butyramide hydrolysis was 2 to 3% of the rate of acetamide hydrolysis for all species including those strains able to grow on butyramide. The amidases of P. putida a87 and P. acidovorans 14 were inducible by butyramide. Cell-free extracts of P. aeruginosa strains induced with acetamide cross-reacted completely with antiserum prepared against purified P. aeruginosa amidase A. Partial cross-reactions occurred with P. putida and P. acidovorans amidases and a very weak cross-reaction with P. cepacia amidase. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-71-2-241