RT Journal Article SR Electronic(1) A1 HUMPHREYS, G. O. A1 HANCOCK, I. C. A1 MEADOW, PAULINE M.YR 1972 T1 Synthesis of the Hydroxyacids in Lipid A of Pseudomonas aeruginosa JF Microbiology, VO 71 IS 2 SP 221 OP 230 DO https://doi.org/10.1099/00221287-71-2-221 PB Microbiology Society, SN 1465-2080, AB Summary Washed suspensions and particulate fractions of Pseudomonas aeruginosa paci incorporated radioactivity from [1-14C]decanoic acid into bound 3-hydroxydecanoate, and from [1-14C]dodecanoate into bound 2- and 3-hydroxydodecanoate. Washed suspensions incorporated [1-14C]acetate into all three hydroxyacids, which are components of Lipid A in this organism. Degradation studies suggested that 2-hydroxydodecanoate was synthesized by direct hydroxylation of dodecanoate. Coenzyme A was required for synthesis of both 2- and 3-hydroxydodecanoates, synthesis of the former being stimulated by pyridine nucleotide coenzymes. Coenzyme A could not be replaced by acyl carrier protein which inhibited synthesis, but it was not required if [1-14C]dodecanoyl Coenzyme A was used as substrate. The hydroxyacids appeared to be incorporated into lipopolysaccharide since the radioactivity incorporated was extracted by hot 45& phenol and labelled hydroxyacids were then released only after acid or alkali hydrolysis., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-71-2-221