1887

Abstract

SUMMARY: Rapid fusion after contact between multinucleate, naturally synchronous plasmodia of the same strain of was demonstrated by autoradiography. Fusion between plasmodia of equal size at the same stage in the mitotic cycle resulted in synchronous mitosis with a delay. This delay was slight if the nuclei were about to undergo mitosis at the time of fusion, but increased in a roughly linear fashion to about 4 h in plasmodia fused at an early stage in the mitotic cycle. Fusion between plasmodia at different stages in the mitotic cycle also resulted in synchronous mitosis, division of nuclei late in the cycle being delayed and of those early in the cycle being accelerated. When both plasmodia were in mid-interphase, the amount of acceleration was proportional to the difference in stages at the time of fusion, a 5 h phase difference giving about 3 h acceleration. However, non-synchronous mitosis resulted when a plasmodium about to undergo mitosis was fused with one earlier in the nuclear cycle, the nuclei in the former plasmodium presumably having reached a stage at which retardation could not occur.

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/content/journal/micro/10.1099/00221287-71-1-93
1972-06-01
2021-08-04
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References

  1. CARLILE M. J., DEE J. 1967; Plasmodial fusion and lethal interaction between strains in a myxomycete.. Nature, London 215:832–834
    [Google Scholar]
  2. CHIN B., BERNSTEIN I. A. 1968; Adenosine triphosphate and synchronous mitosis in Physarum poly- cephalum.. Journal of Bacteriology 96:330–337
    [Google Scholar]
  3. GUTTES S., GUTTES E. 1968; Regulation of DNA replication in the nuclei of the slime mold Physarum polycephalum. Transplantation of nuclei by plasmodial coalescence.. Journal of Cell Biology 37:761–772
    [Google Scholar]
  4. HARRIS H. 1970 Cell Fusion.. Cambridge, Massachusetts:: Harvard University Press.;
    [Google Scholar]
  5. HOWARD A., PELC S. T. 1953 Synthesis of deoxyribonucleic acid in normal and irradiated cells and its relation to chromosome breakage.. In Symposium on Chromosome Breakage, Supplement to Heredity, vol VI p. 261. Edited by Darlington. C. D. Springfield:: Charles C. Thomas.;
    [Google Scholar]
  6. HOWARD F. L. 1932; Nuclear division in plasmodium of Physarum.. Annals of Botany 46:461–477
    [Google Scholar]
  7. NYGAARD O., , GUTTES S., RUSCH H. P. 1960; Nucleic acid metabolism in a slime mold with synchronous mitosis.. Biochimica et biophysica acta 38:298–306
    [Google Scholar]
  8. RAO B., GONTCHAROFF M. 1969; Functionality of newly-synthesized DNA as related to RNA synthesis during the mitotic cycle in Physarum polycephalum.. Experimental Cell Research 56:269–274
    [Google Scholar]
  9. Rao P. N., JOHNSON R. T. 1970; Mammalian cell fusion: studies on the regulation of DNA synthesis and mitosis.. Nature, London 225:159–164
    [Google Scholar]
  10. RUSCH H. P. 1970; Some biochemical events in the life-cycle of Physarum polycephalum.. In Advances in Cell Biology,. vol 1 pp. 297–327 Edited by Prescott D. M., Goldstein L., McConkey. E. New York:: Appleton-Century-Crofts.;
    [Google Scholar]
  11. RUSCH H. P., SACHSENMAIER W., BEHRENS K., GRUTER V. 1966; Synchronization of mitosis by the fusion of the plasmodia of Physarum polycephalum.. Journal of Cell Biology 31:204–209
    [Google Scholar]
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