1887

Abstract

SUMMARY: Simple chemical analyses were carried out on 198 nocardioform bacteria by means of paper- and thin-layer chromatography. The strains considered to belong to the genus contained the lipid LCN-A, arabinose and -diaminopimelic acid. All the representative strains from the complex possessed this lipid though in certain cases the characteristic spot had a slightly lower value than that of the reference lipid LCN-A from the standard strain of The genera and did not contain lipid LCN-A and the distribution of the other two chemical characters varied. The method used to detect lipid LCN-A is simple and reliable and permits the separation of nocardias and strains from allied taxa. These results correlate well with other trends in the taxonomy of nocardioform bacteria and confirm the value of chemotaxonomic characters, especially lipids, in the classification and identification of these organisms.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-71-1-77
1972-06-01
2024-10-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/71/1/mic-71-1-77.html?itemId=/content/journal/micro/10.1099/00221287-71-1-77&mimeType=html&fmt=ahah

References

  1. ASSELINEAU J. 1966 The Bacterial Lipids.. Paris:: Hermann.;
    [Google Scholar]
  2. BECKER B., LECHEVALIER M. P., GORDON R. E., LECHEVALIER H. A. 1964; Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates.. Applied Microbiology 12:421–423
    [Google Scholar]
  3. BECKER B., LECHEVALIER M. P., LECHEVALIER H. A. 1965; Chemical composition of cell-wall preparations from strains of various form-genera of aerobic actinomycetes.. Applied Microbiology 13:236–243
    [Google Scholar]
  4. ETÉMADI A. H. 1967a; Structural and biogenetic correlations in mycolic acids in relation to the phylogenesis of Actinomycetales genera.. Bulletin de la Société de chimie biologique 49:695–706
    [Google Scholar]
  5. ETÉMADI A. H. 1967b; The use of pyrolysis gas chromatography and mass spectroscopy in the study of the structure of mycolic acids.. Journal of Gas Chromatography 5:447–456
    [Google Scholar]
  6. GOODFELLOW M. 1971; Numerical taxonomy of some nocardioform bacteria.. Journal of General Microbiology 69:33–80
    [Google Scholar]
  7. GOODFELLOW M., FLEMING A., SACKIN M. J. 1971; Differentiation of Mycobacterium rhodochrous and Runyon’s Group IV mycobacteria using numerical taxonomic techniques.. Journal of General Microbiology,viii
    [Google Scholar]
  8. Gordon R. E. 1966; Some strains in search of a genus – Corynebacterium, Mycobacterium, Nocardia or what?. Journal of General Microbiology 43:329–343
    [Google Scholar]
  9. LANÉELLE M. A., ASSELINEAU J., CASTELNUOVO G. 1965; Études sur les mycobacteries et les nocardiae. IV. Composition des lipides de Mycobacterium rhodochrous M. pellegrino sp., et de quelques souches de nocardiae.. Annales de l’Institut Pasteur 108:69–82
    [Google Scholar]
  10. LECHEVALIER M. P., LECHEVALIER H. A. 1970a; Chemical composition as a criterion in the classification of aerobic actinomycetes.. International Journal of Systematic Bacteriology 20:435–444
    [Google Scholar]
  11. LECHEVALIER H. A., LECHEVALIER M. P. 1970b A critical evaluation of the genera of aerobic actinomycetes.. In The Actinomycetales, pp. 393–405 Edited by Prauser H. Jena:: Gustav Fischer.;
    [Google Scholar]
  12. LECHEVALIER M. P., HORAN A. C., LECHEVALIER H. A. 1971; Lipid composition in the classification of nocardiae and mycobacteria.. Journal of Bacteriology 105:313–318
    [Google Scholar]
  13. MORDARSKA H. 1968; A trial of using lipids for the classification of actinomycetes.. Archivum immunologiae et therapiae experimentalis 16:45–50
    [Google Scholar]
  14. MORDARSKA H., MORDARSKI M. 1969; Comparative studies on the occurrence of lipid A, diaminopimelic acid and arabinose inNocardia cells.. Archivum immunologiae et therapiae experimentalis 17:739–743
    [Google Scholar]
  15. MORDARSKA H., MORDARSKI M. 1970 Cell lipids of Nocardia.. In The Actinomycetales, pp. 47–53 Edited by Prauser H. Jena:: Gustav Fischer.;
    [Google Scholar]
  16. MORDARSKA H., Rethy A. 1970; Preliminary studies on the chemical character of the lipid fraction in Nocardia.. Archivum immunologiae et therapiae experimentalis 18:455–459
    [Google Scholar]
  17. MORDARSKI M., WIECZOREK J., JAWORSKA B. 1970; On the conditions of amylase production by actinomycetes.. Archivum immunologiae et therapiae experimentalis 18:375–381
    [Google Scholar]
  18. MURRAY I. G., PROCTOR A. G. J. 1965; Paper chromatography as an aid to the identification of Nocardia species.. Journal of General Microbiology 41:163–167
    [Google Scholar]
  19. PRAUSER H. 1967 Contributions to the taxonomy of the Actinomycetales.. Publication of the Faculty of Sciences; J. E. Purkyne University, Brno K: 40196–199
    [Google Scholar]
  20. PRAUSER H., FALTA R. 1968; Phagensensibilitat, Zellwand-Zusammensetzung und Taxonomie von Actinomyceten.. Zeitschrift fiir allgemeine Mikrobiologie 8:39–46
    [Google Scholar]
  21. PRAUSER H., LECHEVALIER M. P., LECHEVALIER H. A. 1970; Description of Oerskovia gen. n. to harbor Orskov’s motile Nocardia.. Applied Microbiology 19:534
    [Google Scholar]
  22. SNEATH P. H. A., SKERMAN V. B. D. 1966; A list of type and reference strains of bacteria.. International Journal of Systematic Bacteriology 16:1–133
    [Google Scholar]
  23. ŠUPUT J., LECHEVALIER M. P., LECHEVALIER H. A. 1967; Chemical composition of variants of aerobic actinomycetes.. Applied Microbiology 15:1356–1361
    [Google Scholar]
  24. WAKSMAN S. A. 1957 In Berge’s Manual of Determinative Bacteriology,. , 7th edn.. Baltimore:: Williams & Wilkins.;
    [Google Scholar]
/content/journal/micro/10.1099/00221287-71-1-77
Loading
/content/journal/micro/10.1099/00221287-71-1-77
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error