SUMMARY: The intra- and extracellular concentrations of a number of phosphorylated intermediary metabolites in were measured radiochemically during growth in low phosphate (0.75 mM) media containing [P]P. Distribution studies showed that most of the monophosphate metabolites were present in the supernatant medium outside the bacteria. This was much less marked for the di- and triphosphates, and was not due to bacterium lysis. Adenosine 3′,5′-cyclic monophosphate was present solely in the medium under all conditions tested. 3′,5′-Cyclic-AMP added to the medium was only partly effective in reversing repression of β-galactosidase synthesis by gluconate. This was not due to degradation of the nucleotide.

In another series of strains, chemical determinations were made of the intra-and extracellular concentrations of fructose 1,6-diphosphate and triose phosphate under varying conditions of growth in high phosphate (0.1 M) media; while most of the fructose 1,6-diphosphate remained inside the bacteria, triose phosphate was present largely in the medium. In all of these studies there was no correlation between the severity of catabolite repression and the concentrations of metabolites either inside the bacteria, or in the medium.


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