1887

Abstract

SUMMARY: Pentachloronitrobenzene (PCNB)-sensitive and PCNB-resistant strains of were grown in liquid and on solid media with and without PCNB. On solid medium with PCNB, hyphae of the sensitive strain had thick walls, were shorter, branched less and had fewer nuclei per cell compared with the control. Under these conditions resistant hyphae were normal and less affected in growth parameters. In liquid medium the effect of PCNB on growth was less marked, but production of extracellular polysaccharide was decreased in the sensitive strain but not in the resistant one. The walls of resistant hyphae had a low hexosamine content compared with those of the wild-type; it increased in both by about a third in the presence of PCNB. Sodium deoxycholate had no effect on it. There was no difference in alkali-soluble material between the strains; it was decreased in both in the presence of PCNB, but more so in the sensitive than in the resistant strain. Changes in carbohydrate synthesis were a result of PCNB action on the sensitive wild-type; the resistant mutant withstood similar changes without such gross changes in morphology.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-71-1-173
1972-06-01
2022-05-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/71/1/mic-71-1-173.html?itemId=/content/journal/micro/10.1099/00221287-71-1-173&mimeType=html&fmt=ahah

References

  1. COHEN J., KATZ D., ROSENBERGER R. F. 1969 Temperature-sensitive mutant of Aspergillus nidulans lacking amino sugars in its cell wall.. Nature, London 224713–715
    [Google Scholar]
  2. CROCKEN B., TATUM E. L. 1968; The effect of sorbose on metabolism and morphology of Neurospora.. Biochimica et biophysica acta 156:1–8
    [Google Scholar]
  3. Dische Z., Borenfreund E. 1950; A spectrophotometric method for the microdetermination of hexos-amines.. Journal of Biological Chemistry 184:517–522
    [Google Scholar]
  4. Distler J. J., Roseman S. 1960; Galactosamine polymers produced by Aspergillus parasiticus.. Journal of Biological Chemistry 235:2538–2541
    [Google Scholar]
  5. DUBOIS M., GILLES K. A., HAMILTON J. K., REBERS P. A., SMITH F. 1956; Colorimetric method for the determination of sugars and related substances.. Analytical Chemistry 28:350–356
    [Google Scholar]
  6. EVELEIGH D. E., GORIN P. A. J. 1969; An extracellular polysaccharide containing N-acetylgalactos- amine and galactose units from Aspergillus nidulans.. Aspergillus Newsletter 10:25
    [Google Scholar]
  7. FREY R. 1950; Chitin undZellulose in Pilzzellw#x00E4;nden.. Bericht der Schweizerischen botanischen Gesellschaft 60:199–230
    [Google Scholar]
  8. Gorin P. A. J., Eveleigh D. E. 1970; Extracellular 2-acetamido-2-deoxy-D-galacto-D-galactan from Aspergillus nidulans.. Biochemistry 9:5023–5027
    [Google Scholar]
  9. HAROLD F. M. 1962; Binding of inorganic polyphosphate to the cell wall of Neurospora crassa.. Biochimica et biophysica acta 57:59–66
    [Google Scholar]
  10. JOHNSTON I. R. 1963; The determination of galactosamine in the presence of glucosamine.. Biochemical Journal 86:254–258
    [Google Scholar]
  11. Johnston I. R. 1965; The composition of the cell wall of Aspergillus niger.. Biochemical Journal 96:651–658
    [Google Scholar]
  12. Livingston L. R. 1969; Locus-specific changes in cell wall composition characteristic of osmotic mutants of Neurospora crassa.. Journal of Bacteriology 99:85–90
    [Google Scholar]
  13. Mackintosh M. E., Pritchard R. H. 1963; The production and replica plating of microcolonies of Aspergillus nidulans.. Genetical Research 4:320–322
    [Google Scholar]
  14. MAKRIS B., GEORGOPOULOS S. G. 1969; Reduced hexosamine content of fungal cell wall due to the fungicide pentachloronitrobenzene.. Phytopathology 59:879–880
    [Google Scholar]
  15. Reavill M. J. 1954; Effect of certain chloronitrobenzenes on germination, growth and sporulation of some fungi.. Annals of Applied Biology 41:448–460
    [Google Scholar]
  16. RIZVI S. R. H., ROBERTSON N. F. 1965; Apical disintegration of hyphae as a response to L-sorbose.. Transactions of the British Mycological Society 48:469–477
    [Google Scholar]
  17. de Terra Terra., Tatum E. L. 1961 Colonial growth of Neurospora.. Science, New York 1341066–1068
    [Google Scholar]
  18. Threlfall R. J. 1968; The genetics and biochemistry of mutants of Aspergillus nidulans resistant to chlorinated nitrobenzenes.. Journal of General Microbiology 52:35–44
    [Google Scholar]
  19. Trevithick J. R., Metzenberg R. L. 1966; Genetic alteration of pore size and other properties of the Neurospora cell wall.. Journal of Bacteriology 92:1016–1020
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-71-1-173
Loading
/content/journal/micro/10.1099/00221287-71-1-173
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error