1887

Abstract

SUMMARY: Electron micrographs of thin sections of cells of the mutantof showed that it, like artificially prepared protoplasts, possessed no cell wall. Invertase was produced by the mutant and appeared to be serologically identical to wild-type invertase. The mutant secreted over 95 % of theinvertase into the medium whereas cells of the wild-type retained almost all the invertase. The mutant hence resembles wild-type protoplasts in both structure and invertase secretion.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-71-1-159
1972-06-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/71/1/mic-71-1-159.html?itemId=/content/journal/micro/10.1099/00221287-71-1-159&mimeType=html&fmt=ahah

References

  1. BACHMANN B. J., BONNER D. M. 1959; Protoplasts from Neurospora crassa.. Journal of Bacteriology 78:550–556
    [Google Scholar]
  2. BATES W. K., HEDMAN S. C., WOODWARD D. O. 1967; Comparative inductive responses of two β-galactosidases of Neurospora.. Journal of Bacteriology 93:1631–1637
    [Google Scholar]
  3. CHUNG P. Y., TREVITHICK J. R. 1970; Biochemical and histochemical localization of invertase in Neurospora crassa during conidial germination and hyphal growth.. Journal of Bacteriology 102:423–429
    [Google Scholar]
  4. EMERSON S. 1963; Slime, a plasmodioid variant of Neurospora crassa.. Genetica 34:162–182
    [Google Scholar]
  5. GRATZNER H., SHEEHAN D. N. 1969; Neurospora mutant exhibiting hyperproduction of amylase and invertase.. Journal of Bacteriology 97:544–549
    [Google Scholar]
  6. HIRANO T., TACREITER W., EAVES A., KAPLAN J. G. 1968; The plasma membrane of yeast protoplasts.. Cytologia 33:558–564
    [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  8. Manocha M. S. 1968; Electron microscopy of the conidial protoplasts of Neurospora crassa.. Canadian Journal of Botany 46:1561–1564
    [Google Scholar]
  9. MANOCHA M. S., COLVIN J. R. 1967; Structure and composition of the cell wall of Neurospora crassa.. Journal of Bacteriology 94:202–212
    [Google Scholar]
  10. MEACHUM Z. D., BRAYMER H. D. 1969; Localization of invertase in the cell wall of Neurospora crassa.. Proceedings of the 158th National Meeting of the American Chemical Society, Abstract no. 195
    [Google Scholar]
  11. MEACHUM Z. D., COLVIN H. J., BRAYMER H. D. 1971; Chemical and physical studies of Neurospora crassa invertase; molecular weight, amino acid and carbohydrate composition and quaternary structure.. Biochemistry 10:326–332
    [Google Scholar]
  12. METZENBERG R. L. 1962; A gene affecting the repression of invertase and trehalase in Neurospora.. Archives of Biochemistry and Biophysics 96:468–474
    [Google Scholar]
  13. METZENBERG R. L. 1963; The localization of β-fructofuranosidase in Neurospora.. Biochimica et biophysica acta 77:455–465
    [Google Scholar]
  14. NAMBOODIRI A. N. 1966; Electron microscopic studies on the conidia and hyphae of Neurospora crassa.. Caryologia 19:117–132
    [Google Scholar]
  15. Ouchterlony O. 1949; Antigen-antibody reactions in gels.. Actapathologica et microbiologica scandinavica 26:507–513
    [Google Scholar]
  16. REYNOLDS E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.. Journal of Cell Biology 17:208–213
    [Google Scholar]
  17. SARGENT M. L., WOODWARD D. O. 1969; Gene-enzyme relationships in Neurospora invertase.. Journal of Bacteriology 97:867–872
    [Google Scholar]
  18. SHATKIN A. J., TATUM E. L. 1959; Electron microscopy of Neurospora crassa mycelia.. Journal of Biophysical and Biochemical Cytology 6:423–426
    [Google Scholar]
  19. TREVITHICK J. R., METZENBERG R. L. 1964; The invertase isozyme formed by Neurospora protoplasts.. Biochemical and Biophysical Research Communications 16:319–325
    [Google Scholar]
  20. TREVITHICK J. R., METZENBERG R. L. 1966; Molecular sieving by Neurospora cell walls during secretion of invertase isozymes.. Journal of Bacteriology 92:1010–1015
    [Google Scholar]
  21. VOGEL H. J. 1964; Distribution of lysine pathways among fungi; evolutionary implications.. American Naturalist 98:435–436
    [Google Scholar]
  22. VAN WINCKLE W. B. 1969; Preliminary observations of the ultrastructure of the slime mutant.. Neurospora Newsletter 14:5
    [Google Scholar]
  23. WOODWARD V. W., WOODWARD C. K. 1968; The care and feeding of slime.. Neurospora Newsletter 13:18
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-71-1-159
Loading
/content/journal/micro/10.1099/00221287-71-1-159
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error