1887

Abstract

SUMMARY: Many monosaccharides, oligosaccharides and their derivatives were tested as inhibitors of agglutination of guinea-pig or horse erythrocytes by or bacteria bearing type-1 fimbriae. D-Mannose and certain derivatives of mannose were strongly inhibitory, and D-fructose moderately inhibitory. Modification of any of the hydroxyl groups at the C-2, C-3, C-4 or C-6 positions in the D-mannopyranosyl molecule caused failure to inhibit, showing that these groups were necessary for binding to the activesites of the fimbrial haemagglutinin. The α-configuration at the C-1 position in D-mannose was important and carbohydrates containing α-linked D-mannose were much more active inhibitors than those containing β-linked D-mannose, which were poor inhibitors or non-inhibitors. Yeast mannan also inhibited the fimbrial reaction. Attention is drawn to the remarkable degree of similarity ofthe inhibition pattern of the type-1 fimbrial haemagglutinin of bacteria with that of concanavalin-A, the jack bean haemagglutinin.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-71-1-149
1972-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/71/1/mic-71-1-149.html?itemId=/content/journal/micro/10.1099/00221287-71-1-149&mimeType=html&fmt=ahah

References

  1. BRINTON C. C. (1965); The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in Gram-negative bacteria.. Transactions of the New York Academy of Sciences 27:1003–1054
    [Google Scholar]
  2. Collier W. A., Jacoeb M. 1955; Bacterien-Haemagglutination. I. Versuche mit einem haemagglutin-erenden Stamm von E. coli.. Antonie van Leeuwenhoek 21:113–123
    [Google Scholar]
  3. COLLIER W. A., DE MIRANDA J. C. 1955; Bacterien-Haemagglutination. III. Die Hemmung der Coli-Haemagglutination durch Mannose.. Antonie van Leeuwenhoek 21:133–140
    [Google Scholar]
  4. Collier W. A., Tiggelman-van Krugten V. A. H. 1957; Bacterien-Haemagglutination. V. Weitere Versuche iiber Hemmung der Coli-Haemagglutination durch chemische Stoffe.. Antonie van Leeuwenhoek 23:289–304
    [Google Scholar]
  5. COLLIER W. A., TIGGELMAN-VAN KRUGTEN V. A. H., TJONG A HUNG T. 1955; Bacterien-Haemagglutina- tion. IV. Ueber Haemagglutinationsversuche bei 105 Colistammen und Adsorption der Haemag- glutinine.. Antonie van Leeuwenhoek 21:303–315
    [Google Scholar]
  6. Duguid J. P. 1959; Fimbriae and adhesive properties in Klebsiella strains.. Journal of General Microbiology 21:271–286
    [Google Scholar]
  7. Duguid J. P., Anderson E. S., Campbell I. 1966; Fimbriae and adhesive properties in salmonellae.. Journal of Pathology and Bacteriology 92:107–137
    [Google Scholar]
  8. DUGUID J. P., CAMPBELL I. 1969; Antigens of the type-1 fimbriae of salmonellae and other enterobacteria.. Journal of Medical Microbiology 2:535–553
    [Google Scholar]
  9. DUGUID J. P., GILLIES R. R. 1957; Fimbriae and adhesive properties in dysentery bacilli.. Journal of Pathology and Bacteriology 74:397–411
    [Google Scholar]
  10. Duguid J. P., Smith I. W., Dempster G., Edmunds P. N. 1955; Non-flagellar filamentous appendages ('fimbriae') and haemagglutinating activity in Bacterium coli.. Journal of Pathology and Bacteriology 70:335–348
    [Google Scholar]
  11. GOLDSTEIN I. J., HOLLERMAN C. E., SMITH E. E. 1965; Protein-carbohydrate interaction. II. Inhibition studies on the interaction of concanavalin A with polysaccharides.. Biochemistry 4:876–883
    [Google Scholar]
  12. HEUMANN W., MARX R. 1964; Feinstruktur und Funktion der Fimbrien bei dem sternbildenden Bak-terium Pseudomonas echinoides.. Archiv fur Mikrobiologie 47:325–337
    [Google Scholar]
  13. JONES G. H., BALLOU C. E. 1968; Isolation of an α-mannosidase which hydrolyzes yeast mannan. Structure of the backbone of yeast mannan.. Journal of Biological Chemistry 243:2442–2446
    [Google Scholar]
  14. OLD D. C. 1963 Fimbriation in enterobacteria Ph.D. Thesis University of Edinburgh.;
    [Google Scholar]
  15. OLD D. C., CORNEIL I., GIBSON L. F., THOMSON A. D., DUGUID J. P. 1968; Fimbriation, pellicle formation and the amount of growth of salmonellas in broth.. Journal of General Microbiology 51:1–16
    [Google Scholar]
  16. OLD D. C., DUGUID J. P. 1970; Selective outgrowth of fimbriate bacteria in static liquid medium.. Journal of Bacteriology 103:447–456
    [Google Scholar]
  17. OLD D. C., DUGUID J. P. 1971; Selection of fimbriate transductants of Salmonella typhimurium dependent on motility.. Journal of Bacteriology 107:655–658
    [Google Scholar]
  18. Old D. C., PAYNE S. B. 1971; Antigens of the type-2 fimbriae of salmonellae: ‘cross-reacting material’ (CRM) of type-1 fimbriae.. Journal of Medical Microbiology 4:215–225
    [Google Scholar]
  19. SHEDDEN W. I. H. 1962; Fimbriae and haemagglutinating activity in strains of Proteus hauseri.. Journal of General Microbiology 28:1–7
    [Google Scholar]
  20. SMITH E. E., GOLDSTEIN I. J. 1967; Protein-carbohydrate interaction. V. Further inhibition studies directed toward defining the stereochemical requirements of the reaction sites of concanavalin A.. Archives of Biochemistry and Biophysics 121:88–95
    [Google Scholar]
  21. So L. L., GOLDSTEIN I. J. 1968; Protein-carbohydrate interaction. XIII. The interaction of concanavalin A with a-mannans from a variety of microorganisms.. Journal of Biological Chemistry 243:2003–2007
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-71-1-149
Loading
/content/journal/micro/10.1099/00221287-71-1-149
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error