1887

Abstract

SUMMARY: Washed suspensions of grown and incubated anaerobically engulfed all the types of bacteria tested. At high bacterial densities and two bacteria normally present in the growth medium, were taken up most rapidly. utilized bacterial amino acids for the synthesis of protein and no evidence was obtained for appreciable synthesis of protein from carbohydrate. No intercpnversion of amino acids occurred. Free and bacterial adenine, guanine and uracil were incorporated into protozoal nucleic acid: uracil was partially converted into cytosine. Free amino acids and glucose were taken up by the protozoa by two mechanisms, one predominant at high concentrations and the other at low concentrations. Glucose was incorporated, in intact protozoa, into both polysaccharide and bacteria and glucose had to be taken up by the protozoa before it was incorporated into the bacteria.

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1972-06-01
2024-04-25
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References

  1. Bryant M. P., Robinson I. M. 1963; Apparent incorporation of ammonia and amino acid carbon during growth of selected species of ruminal bacteria.. Journal of Dairy Science 46:150–154
    [Google Scholar]
  2. Christiansen W. C., Kawashima R., Burroughs W. 1965; Influence of protozoa upon rumen acid production and live weight gains in lambs.. Journal of Animal Science 24:730–734
    [Google Scholar]
  3. COLEMAN G. S. 1956; The dissimilation of amino acids by Rhodospirillum rubrum.. Journal of General Microbiology 15:248–256
    [Google Scholar]
  4. COLEMAN G. S. 1958; Maintenance of oligotrich protozoa from sheep rumen in vitro.. Nature, London 182:1104–1105
    [Google Scholar]
  5. COLEMAN G. S. 1960; Effect of penicillin on the maintenance of rumen oligotrich protozoa.. Nature, London 187:518–520
    [Google Scholar]
  6. COLEMAN G. S. 1964; The metabolism of Escherichia coli and other bacteria by Entodinium caudatum.. Journal of General Microbiology 37:209–223
    [Google Scholar]
  7. Coleman G. S. 1967a; The metabolism of free amino acids by washed suspensions of the rumen ciliate Entodinium caudatum.. Journal of General Microbiology 47:433–447
    [Google Scholar]
  8. Coleman G. S. 1967b; The metabolism of the amino acids of Escherichia coli and other bacteria by the rumen ciliate Entodinium caudatum.. Journal of General Microbiology 47:449–464
    [Google Scholar]
  9. Coleman G. S. 1968; The metabolism of bacterial nucleic acid and of free components of nucleic acid by the rumen ciliate Entodinium caudatum.. Journal of General Microbiology 54:83–96
    [Google Scholar]
  10. COLEMAN G. S. 1969a; The cultivation of the rumen ciliate Entodinium simplex.. Journal of General Microbiology 57:81–90
    [Google Scholar]
  11. COLEMAN G. S. 1969b; The metabolism of starch, maltose, glucose and some other sugars by the rumen ciliate Entodinium caudatum.. Journal of General Microbiology 57:303–332
    [Google Scholar]
  12. COLEMAN G. S., HALL F. J. 1969; Electron microscopy of the rumen ciliate Entodinium caudatum with special reference to the engulfment of bacteria and other particulate matter.. Tissue and Cell 1:607–618
    [Google Scholar]
  13. EADIE J. M., MANN S. O. 1970; Development of the rumen microbial population: high starch diets and instability.. In Physiology of Digestion and Metabolism in the Ruminant pp. 335:347 Newcastle-upon- Tyne : Oriel Press;
    [Google Scholar]
  14. GUTIERREZ J., WILLIAMS P. P., DAVIS R. E., WARWICK E. J. 1962; Lipid metabolism of rumen ciliates and bacteria. I. Uptake of fatty acids by Isotricha prostomaand Entodinium simplex.. Applied Microbiology 10:548–551
    [Google Scholar]
  15. HUGGETT A. St G., NIXON D. A. 1957; Use of glucose oxidase, peroxidase and o-dianisidine in the determination of blood and urinary glucose.. Lancet ii 368:370
    [Google Scholar]
  16. HUNGATE R. E. 1966 The Rumen and its Microbes. New York and London: Academic Press;
    [Google Scholar]
  17. JARVIS B. D. W., HUNGATE R. E. 1968; Factors influencing agnotobiotic culture of the rumen ciliate Entodinium simplex.. Applied Microbiology 16:1044–1052
    [Google Scholar]
  18. MCNAUGHT M. L., OWEN E. C., HENRY K. M., KON S. K. 1954; The utilization of non-protein nitrogen in the bovine rumen. 8. The nutritive value of the proteins of preparations of dried rumen bacteria, rumen protozoa and brewers yeast for rats.. Biochemical Journal 56:151–156
    [Google Scholar]
  19. PURSER D. B., MOIR R. J. 1966; Dietary effects upon concentrations of protozoa in the rumen.. Journal of Animal Science 25:668–674
    [Google Scholar]
  20. ROBERTS R. B., ABELSON P. H., COWIE D. E., BOLTON E. T., BRITTEN R. J. 1955; Studies on Biosynthesis in Escherichia coli.. Publication of the Carnegie Institution607 Washington: Carnegie Institution;
    [Google Scholar]
  21. WHITE R. W. 1969; Viable bacteria inside the rumen ciliate Entodinium caudatum.. Journal of General Microbiology 56:403–408
    [Google Scholar]
  22. WILLIAMS P. P., GUTIERREZ J., Davis R. E. 1963; Lipid metabolism of rumen ciliates and bacteria. 2. Uptake of fatty acids and lipid analysis of Isotricha intestinalis and rumen bacteria with further information on Entodinium simplex.. Applied Microbiology 11:260–264
    [Google Scholar]
  23. WYATT G. R. 1951; The purine and pyrimidine composition of deoxypentose nucleic acid.. Biochemical Journal 48:584–590
    [Google Scholar]
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