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Volume 71, Issue 1

Nitrogenase Activity and Oxygen Sensitivity of the Paspalum notatum-Azotobacter paspali Association Free

Abstract

SUMMARY: Nitrogenase activity in the rhizosphere of a grass, and its associated soil was measured by the reduction of acetylene. Roots of the cultivar ‘batatais’ colonized by when taken from the soil, produced 1 to 32 nmol CH/g dry wt/h, whereas the cultivar ‘pensacola’, which is not colonized by produced less than 0.5 nmol/g/h. There was a lag of 12 to 24 h before maximum, linear, rates of acetylene reduction were reached. Activity was almost completely inhibited in air or in the absence of O and was greatest at around pO 0.04 atm. Activity of soil cores containing plants with leaves attached was little affected by pO and showed no lag. Soil-plant cores maintained in a 16 h day+8 h night showed no diurnal fluctuation in activity; as the dark period was extended, activity decreased but was restored on returning plants to the light. Roots and rhizomes had most activity, the soil very little and aerial parts none. Washing the roots removed less than half the activity. Disturbance of soil-plant cores decreased activity. The soil next to the root surface contained most more were associated with active plants than with less active plants. Sections of roots showed abundant bacteria adjacent to the root surface. Nitrogen fixation by the association was estimated to be up to 90 kg N/ha/annum.

  • Accepted:
  • Published Online:
© Society for General Microbiology, 1962
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1972-06-01
2023-09-25
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References

  1. ABD-EL-MALEK Y. 1971 Free living nitrogen fixing bacteria in Egyptian soils and their possible contribution to soil fertility.. In Biological Nitrogen Fixation in Natural and Agricultural Habitats. Plant and Soil, special volumepp. 832–834 Edited by Lie T. A., Mulder. E. G. The Hague:: Nyhoff.;
     [Google Scholar]
  2. BILLSON S., WILLIAMS K., POSTGATE J. R. 1970; A note on the effect of diluents on determination of viable numbers of Azotobacteriaceae.. Journal of Applied Bacteriology 33:832–834
     [Google Scholar]
  3. BROUZES R., MAYFIELD C. I., KNOWLES R. 1971 Acetylene and N15 studies in agricultural and forest soils: effect of oxygen partial pressure on nitrogen fixation and acetylene reduction in a sandy loam soil amended with glucose.. In Biological Nitrogen Fixation in Natural and Agricultural Habitats. Plant and Soil, special volume pp. 832–834 Edited by Lie T. A., Mulder. E. G. The Hague:: Nyhoff.;
     [Google Scholar]
  4. BURTON G. W. 1948; The method of reproduction in common Bahia grass Paspalum notatum.. Journal of the American Society of Agronomy 40:832–834
     [Google Scholar]
  5. COOPER J. P. 1970; Potential production and energy conversion in temperate and tropical grasses.. Herbage Abstracts 40:1–15
     [Google Scholar]
  6. DALTON H., POSTGATE J. R. 1969 a; Effect of oxygen on growth of Azotobacter chroococcum in batch and continuous cultures.. Journal of General Microbiology 54:832–834
     [Google Scholar]
  7. DALTON H., POSTGATE J. R. 1969b; Growth and physiology of Azotobacter chroococcum in continuous culture.. Journal of General Microbiology 56:832–834
     [Google Scholar]
  8. DOBEREINER J. 1966; Azotobacter paspali sp. n. uma bacteria fixadora de nitrogenio na rizosfera de Paspalum.. Pesquisa Agropecuaria Brasileira 1:832–834
     [Google Scholar]
  9. DOBEREINER J. 1968; Non-symbiotic nitrogen fixation in tropical soils.. Pesquisa Agropecuaria Brazileira 3:832–834
     [Google Scholar]
  10. DOBEREINER J. 1970; Further research on Azotobacter paspali and its variety specific occurrence in the rhizosphere of Paspalum notatum Fliigge.. Zentralblatt fur Bakteriologie, Parasitenkunde (Abteilung 2) 124:832–834
     [Google Scholar]
  11. DOBEREINER J., CAMPELO A. B. 1971 Non-symbiotic nitrogen fixing bacteria in tropical soils.. In Biological Nitrogen Fixation in Natural and Agricultural Habitats. Plant and Soil, special volume pp. 457–470 Edited by Lie T. A., Mulder. E. G. The Hague:: Nyhoff.;
     [Google Scholar]
  12. DOBEREINER J., DAY J. M., DART P. J. 1972; Nitrogenase activity in the rhizosphere of sugar cane and some other tropical grasses.. Plant and Soil, (in the press).
     [Google Scholar]
  13. DROZD J., POSTGATE J. R. 1970a; Interference by oxygen in the acetylene reduction test for aerobic nitrogen-fixing bacteria.. Journal of General Microbiology 60:832–834
     [Google Scholar]
  14. DROZD J., POSTGATE J. R. 1970b; Effects of oxygen on acetylene reduction, cytochrome content and respiratory activity of Azotobacter chroococcum.. Journal of General Microbiology 63:832–834
     [Google Scholar]
  15. FEDER N., O'BRIEN T. P. 1968; Plant microtechnique: some principles and new methods.. American Journal of Botany 55:832–834
     [Google Scholar]
  16. GREENWOOD D. J. 1971; Studies on the distribution of oxygen around the roots of mustard seedlings Sinapis alba L).. New Phytologist 70:832–834
     [Google Scholar]
  17. HARDY R. W. F., HOLSTEN R. D., JACKSON E. K., BURNS R. C. 1968; The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation.. Plant Physiology 43:832–834
     [Google Scholar]
  18. HATCH M. D., SLACK C. R. 1970; Photosynthetic CO2-fixation pathways.. Annual Review of Plant Physiology 21:832–834
     [Google Scholar]
  19. KASS D. C. 1971 Sources of nitrogen in tropical environments with special reference to the relationship between Paspalum notatum Fliigge and Azotobacter paspali Dobereiner.. M.S. Thesis, Cornell University,; Ithaca, New York.:
     [Google Scholar]
  20. KASS D. C., DROSDOFF M., ALEXANDER M. 1971; Nitrogen fixation by Azotobacter paspali in association with Bahia grass (Paspalum notatum).. American Society for Soil Science Proceedings 35:832–834
     [Google Scholar]
  21. PACEWICZOWA T. H., BALANDREAU J., DOMMERGUES Y. 1970; Fixation microbienne de l'azote dans un sol salin tunesian.. Soil Biology and Biochemistry 2:832–834
     [Google Scholar]
  22. PARKER A. C. 1954; Effect of oxygen on the fixation of nitrogen by Azotobacter.. Nature, London 173:832–834
     [Google Scholar]
  23. PARKER C. A., SCUTT P. B. 1960; The effect of oxygen on nitrogen fixation by Azotobacter.. Biochimica et biophysica acta 38:832–834
     [Google Scholar]
  24. RINAUDO G. 1970 Fixation biologique de Vazote dans trois types de sols de rizieres de cote d'Ivoire.. These de Docteur lngeniuer, Faculte des Sciences,; Montpellier.:
     [Google Scholar]
  25. RINAUDO G., BALANDREAU J., DOMMERGUES Y. 1971 Algal and bacterial non-symbiotic nitrogen fixation in paddy soils.. In Bacterial Nitrogen Fixation in Natural and Agricultural Habitats. Plant and Soil, special volume pp. 832–834 Edited by Lie T. A., Mulder. E. G. The Hague:: Nyhoff.;
     [Google Scholar]
  26. STEYN P. L., DELWICHE C. C. 1970; Nitrogen fixation by non-symbiotic microorganisms in some Cali-fornia soils.. Environmental Science and Technology 4:832–834
     [Google Scholar]
  27. WEINHARD P., JBALANDREAU J., RINAUDO G., DOMMERGUES Y. 1971; Fixation non-symbiotique de l'azote dans la rhizosphere de quelques non-legumineuses tropicales.. Revue d'ecologie et de biologie du sol 8:832–834
     [Google Scholar]
  28. YATES M. G. 1970; Control of respiration and nitrogen fixation by oxygen and adenine nucleotides in N2-grown Azotobacter chroococcum.. Journal of General Microbiology 60:832–834
     [Google Scholar]
  29. YOSHIDA T., ANCAJAS R. R. 1971; Nitrogen fixation by bacteria in the root zone of rice.. Soil Science Society of America Proceedings 35:832–834
     [Google Scholar]
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Nitrogenase Activity and Oxygen Sensitivity of the Paspalum notatum-Azotobacter paspali Association
Microbiology 71, 103 (1972); https://doi.org/10.1099/00221287-71-1-103
/content/journal/micro/10.1099/00221287-71-1-103
/content/journal/micro/10.1099/00221287-71-1-103
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