SUMMARY: Cultures of 15 each newly isolated ethionine-resistant and regulatory mutants of were infected with an F' episome with an amber (UAG) nonsense mutation in the gene. F'Lac ethionine-sensitive derivatives segregating Lac bacteria were obtained from one and one mutant. Genetical and physiological analysis showed that these derivatives had acquired amber suppressors active on the and mutations, leading to the conclusion that the and genes code for polypeptides. The possible roles of the and gene products are discussed in the light of these results.


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