1887

Abstract

SUMMARY: Capsulation of was investigated by genetic transformation with high-molecular-weight deoxyribonucleic acid. The DNA was extracted from a capsulated antibiotic-resistant strain of which had received the type b capsulation locus by transformation. A non-capsulated polyauxotrophic derivative of strain was used as recipient under conditions which permitted the Cap b transformants (assayed as iridescent colonies) to initiate multiplication promptly. The position of on the chromosome map was established by use of chemically defined agar media selective for transformants which had received pairs of linked markers. The locus lies between leucine and biotin loci, and shows about 80 % linkage to the marker. The size of the DNA segment required for Cap b transformation was found by physical methods to be about 33 million daltons.

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/content/journal/micro/10.1099/00221287-70-3-411
1972-05-01
2024-04-20
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References

  1. Alexander H. E., Leidy G. 1951; Determination of inherited traits of H. influenzae by desoxyribonucleic acid fractions isolated from type-specific cells. Journal of Experimental Medicine 93:345–359
    [Google Scholar]
  2. Alexander H. E., Leidy G., Hahn E. 1954; Studies on the nature of Hemophilus influenzae cells susceptible to heritable changes by desoxyribonucleic acids. Journal of Experimental Medicine 99:505–533
    [Google Scholar]
  3. Bendler J. W. III 1968 Size of prophage HPi-ci and amount of DNA between genetic markers in Haemophilus influenzae Doctoral Dissertation The Johns Hopkins University, Baltimore, Maryland:
    [Google Scholar]
  4. Berns K. I., Thomas C. A. Jun 1965; Isolation of high molecular weight DNA from Hemophilus influenzae . Journal of Molecular Biology n:476–490
    [Google Scholar]
  5. Catlin B. W. 1970; Haemophilus influenzae in cultures of cerebrospinal fluid: noncapsulated variants typable by immunofluorescence. American Journal of Diseases of Children 120:203–210
    [Google Scholar]
  6. Catlin B. W., Tartagni V. R. 1969; Delayed multiplication of newly capsulated transformants of Haemophilus influenzae detected by immunofluorescence. Journal of General Microbiology 56:387–401
    [Google Scholar]
  7. Goodgal S. H. 1961; Studies on transformations of Hemophilus influenzae IV. Linked and unlinked transformations. Journal of General Physiology 45:205–228
    [Google Scholar]
  8. Herriott R. M., Meyer E. Y., Vogt M., Modan M. 1970; Defined medium for growth of Haemophilus influenzae . Journal of Bacteriology 101:513–516
    [Google Scholar]
  9. Hershey A. D., Burgi E., Ingraham L. 1963; Cohesion of DNA molecules isolated from phage lambda. Proceedings of the National Academy of sciences of the United States of America 49:748–755
    [Google Scholar]
  10. Jacob F., schaeffer P., Wollman E. L. 1960; Episomic elements in bacteria. Symposium of the Society for General Microbiology 10:67–91
    [Google Scholar]
  11. Leidy G., Hahn E., Zamenhof S., Alexander H. E. 1960; Biochemical aspects OF virulence of Hemophilus influenzae . Annals of the New York Academy of sciences 88:1195–1201
    [Google Scholar]
  12. Mtäkeltä P. H., Stocker B. A. D. 1969; Genetics of polysaccharide biosynthesis. Annual Review of Genetics 3:291–322
    [Google Scholar]
  13. Martin R. G., Ames B. N. 1961; A method for determining the sedimentation behaviour of enzymes: application to protein mixtures. Journal of Biological Chemistry 236:1372–1379
    [Google Scholar]
  14. Michalka J., Goodgal S. H. 1969; Genetic and physical map of the chromosome of Hemophilus influenzae . Journal of Molecular Biology 45:407–421
    [Google Scholar]
  15. Pittman M. 1931; Variation and type specificity in the bacterial species Hemophilus influenzae . Journal of Experimental Medicine 53:471–491
    [Google Scholar]
  16. Rosenberg E., Zamenhof S. 1961; Further studies on polyribophosphate. Journal of Biological Chemistry 236:2845–2849
    [Google Scholar]
  17. Studier F. W. 1965; Sedimentation studies of the size and shape of DNA. Journal of Molecular Biology 11:373–390
    [Google Scholar]
  18. Turk D. C., May J. R. 1967 Haemophilus Influenzae: Its Clinical Importance London: English Universities Press;
    [Google Scholar]
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