SUMMARY: Capsulation of was investigated by genetic transformation with high-molecular-weight deoxyribonucleic acid. The DNA was extracted from a capsulated antibiotic-resistant strain of rd which had received the type b capsulation locus () by transformation. A non-capsulated polyauxotrophic derivative of strain rd was used as recipient under conditions which permitted the Cap b transformants (assayed as iridescent colonies) to initiate multiplication promptly. The position of on the chromosome map was established by use of chemically defined agar media selective for transformants which had received pairs of linked markers. The locus lies between leucine and biotin loci, and shows about 80 % linkage to the marker. The size of the DNA segment required for Cap b transformation was found by physical methods to be about 33 million daltons.


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