The Exopolysaccharides of Klebsiella Serotype 2 Strains as Substrates for Phage-induced Polysaccharide Depolymerases Free

Abstract

SUMMARY: A phage-induced enzyme has been used to hydrolyse the exopolysaccharides prepared from nine Klebsiella serotype 2 strains. In each case, the major product was a tetrasaccharide with chemical composition corresponding to the carbohydrate repeating unit of the polysaccharide. The tetrasaccharides also contained formate, sometimes with acetate or pyruvate. As the terminal reducing sugar in each tetrasaccharide was mannose, the enzyme is a mannosidase hydrolysing the -mannosyl 1 → 4 -glucose linkage.

The enzyme is highly specific, being inactive against carboxyl-reduced type 2 polysaccharide and against polysaccharides from a number of other Klebsiella strains of different serotype. In contrast, similar phage-induced enzymes from serotype 54 strains hydrolyse both type 2 and type 54 polysaccharides, yielding the same products from type 2 material as does the homologous enzyme. No further polysaccharides among those currently tested acted as substrates for the phage-induced enzymes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-70-2-331
1972-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/70/2/mic-70-2-331.html?itemId=/content/journal/micro/10.1099/00221287-70-2-331&mimeType=html&fmt=ahah

References

  1. Adams M.H., Park B.H. 1956; An enzyme produced by a phage-host cell system. II. The properties of the polysaccharide depolymerase. Virology 2:719–736
    [Google Scholar]
  2. Bitter T., Muir H.M. 1962; A modified uronic acid carbazole reaction. Analytical Biochemistry 4:330–334
    [Google Scholar]
  3. Dische Z., Shettles L.B., Osnos M. 1949; New specific colour reactions of hexoses and spectro-photometric micromethods for their determination. Archives of Biochemistry and Biophysics 22:169–186
    [Google Scholar]
  4. Dudman W.F., Wilkinson J.F. 1956; The composition of the extracellular polysaccharide of Aero-bacter-Klebsiella strains. Biochemical Journal 62:289–295
    [Google Scholar]
  5. Eklund C., Wyss O. 1962; Enzyme associated with bacteriophage infection. Journal of Bacteriology 84:1209–1215
    [Google Scholar]
  6. Feather M.S., Whistler R.L. 1962; Isolation and characterisation of the principle hemicellulose from corn germ. Archives of Biochemistry and Biophysics 98:111–115
    [Google Scholar]
  7. Fischer F.G., Dörfel H. 1955; Die papier-chromatographische Trennung and Bestimmung der Uronsauren. Hoppe-Seyler’s Zeitschrift für physiologische Chemie 301:224–234
    [Google Scholar]
  8. Gahan L.C., Sandford P.A., Conrad H.E. 1967; The structure of the serotype 2 capsular polysaccharide of Aerobacter aerogenes . Biochemistry 6:2755–2766
    [Google Scholar]
  9. Gormus B.J., Wheat R.W., Porter J.F. 1971; Occurrence of pyruvic acid in capsular polysaccharides from various Klebsiella species. Journal of Bacteriology 107:150–154
    [Google Scholar]
  10. Grant W.M. 1948; Colorimetric micro-determination of formic acid based on reduction to formaldehyde. Analytical Chemistry 20:267–269
    [Google Scholar]
  11. Hestrin S. 1949; The reaction of acetylcholine and other carboxylic acid derivatives with hydroxylamine and its analytical application. Journal of Biological Chemistry 180:249–261
    [Google Scholar]
  12. Koeltzow D.E., Epley J.D., Conrad H.E. 1968; The lipopolysaccharides of Aerobacter aerogenesstrains A3(si) and NCTC243. Biochemistry 7:2920–2928
    [Google Scholar]
  13. Lawson C.J., Mcleary C.W., Nakada H.I., Rees D.A., Sutherland I.W., Wilkinson J.F. 1969; Structural analysis of colanic acid from Escherichia coli by using methylation and base-catalysed fragmentation. Biochemical Journal 115:947–958
    [Google Scholar]
  14. Lesley S.M. 1961; Degradation of the polysaccharide of Xanthomonasphaseoli by an extracellular bacterial enzyme. Canadian Journal of Microbiology 7:815–825
    [Google Scholar]
  15. Orentas D.G., Sloneker J.H., Jeanes A. 1963; Pyruvic acid content and constituent sugars of exocellular polysaccharides from different species of the genus Xanthomonas . Canadian Journal of Microbiology 9:427–430
    [Google Scholar]
  16. Park S.H., Eriksen J., Henriksen S.D. 1967; Structure of the capsular polysaccharide of Klebsiella pneumoniae type 2 (b). Acta pathologica et microbiologica scandinavica 69:431–436
    [Google Scholar]
  17. Partridge S.M. 1946; Application of the paper partition chromatogram to the qualitative analysis of reducing sugars. Nature; London: 158270–271
    [Google Scholar]
  18. Quayle J.R. 1966; Formate dehydrogenase. In Methods in Enzymology 9 pp 360–364 Colowick S. P., Kaplan N. O. Edited by New York & London: Academic Press;
    [Google Scholar]
  19. Sloneker J.H., Orentas D.G. 1962; Pyruvic acid, a unique component of an exocellular bacterial polysaccharide. Nature; London: 194478–479
    [Google Scholar]
  20. Somogyi M. 1945; A new reagent for the determination of sugars. Journal of Biological Chemistry 160:61–69
    [Google Scholar]
  21. Sutherland I.W. 1967; Phage-induced fucosidases hydrolysing the exopolysaccharide of Klebsiella aerogenes type 54 (A3(si)). Biochemical Journal 104:278–285
    [Google Scholar]
  22. Sutherland I.W. 1970; Formate, a new component of bacterial exopolysaccharides. Nature; London: 228280
    [Google Scholar]
  23. Sutherland I.W. 1971; The occurrence of acyl groups in Klebsiella exopolysaccharides. Journal of General Microbiology 65:v
    [Google Scholar]
  24. Sutherland I.W., Jann K., Jann B. 1970; The isolation of O-acetylated fragments from the K antigen of Escherichia coli 08: K27(A) by the action of phage-induced enzymes from Klebsiella aerogenes . European Journal of Biochemistry 12:285–288
    [Google Scholar]
  25. Sutherland I.W., Wilkinson J.F. 1965; Depolymerases for bacterial polysaccharides obtained from phage-infected bacteria. Journal of General Microbiology 39:373–383
    [Google Scholar]
  26. Sutherland I.W., Wilkinson J.F. 1968; The exopolysaccharide of Klebsiella aerogenes a3(s 1) (type 54). The isolation of O-acetylated octasaccharide, tetrasaccharide and trisaccharide. Biochemical Journal no:749–754
    [Google Scholar]
  27. Thomson A.R. 1951; The separation of saturated mono-hydromaxic acids by partition chromatography on paper. Australian Journal of scientific Research, B 4:180–187
    [Google Scholar]
  28. Watson K.C. 1966; The effect of polysaccharide depolymerising enzyme in gel diffusion and haemag-glutination studies. Immunology 10:121–126
    [Google Scholar]
  29. Whistler R.L., Conrad H.E. 1954; 2-o-(d-Galactopyranobyluronic acid)-l-rhamnose from okra mucilage. Journal of the American Chemical Society 76:3544–3546
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-70-2-331
Loading
/content/journal/micro/10.1099/00221287-70-2-331
Loading

Data & Media loading...

Most cited Most Cited RSS feed