RT Journal Article SR Electronic(1) A1 Orpin, C. G.YR 1972 T1 The Culture of the Rumen Organism Eadie's Oval in vitro JF Microbiology, VO 70 IS 2 SP 321 OP 329 DO https://doi.org/10.1099/00221287-70-2-321 PB Microbiology Society, SN 1465-2080, AB SUMMARY Clone cultures of two strains of the rumen organism Eadie’s Oval were established in vitro. Both strains were maintained anaerobically at 38 to 40° in liquid culture in the presence of bacteria, rumen fluid, a reducing agent and a CO2 gas phase. There was no growth in sterile or solid media. Growth and storage of glycogen occurred when each strain was incubated with one of a small range of simple carbohydrates; amino acids, fatty acids, proteins and polysaccharides did not support growth or glycogen synthesis. Neither strain survived freezing, but cultures were viable after two weeks at 4°; incubation at 44° resulted in no growth or the death of cultures., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-70-2-321