1887

Abstract

SUMMARY

Clone cultures of two strains of the rumen organism Eadie’s Oval were established Both strains were maintained anaerobically at 38 to 40° in liquid culture in the presence of bacteria, rumen fluid, a reducing agent and a CO gas phase. There was no growth in sterile or solid media. Growth and storage of glycogen occurred when each strain was incubated with one of a small range of simple carbohydrates; amino acids, fatty acids, proteins and polysaccharides did not support growth or glycogen synthesis. Neither strain survived freezing, but cultures were viable after two weeks at 4°; incubation at 44° resulted in no growth or the death of cultures.

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/content/journal/micro/10.1099/00221287-70-2-321
1972-04-01
2021-10-24
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