1887

Abstract

SUMMARY

The possession of flagellar hooks by non-flagellate mutants derived from phase 1 stable strains of was determined by electron microscopic examination of material detached from these bacteria by mechanical means. Hooks were found in all the preparations from mutants but none in those from mutants representative of fourteen different complementation groups. The hooks from mutants were morphologically indistinguishable from those of wild-type. These results show that all the genes are involved in hook formation and that the flagellin structural gene is not.

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/content/journal/micro/10.1099/00221287-70-2-299
1972-04-01
2024-04-24
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References

  1. Abram D., Mitchen J.R., Koffler H., Vatter A.E. 1970; Differentiation within the bacterial flagellum and isolation of the proximal hook. Journal of Bacteriology 101:250–261
    [Google Scholar]
  2. Abram D., Vatter A.E., Koffler H. 1965; Basal structure and attachment OF flagella IN cells OF Proteus vulgaris . Journal of Bacteriology 90:1337–1354
    [Google Scholar]
  3. Abram D., Vatter A.E., Koffler H. 1966; Attachment and structural features of flagella of certain bacilli. Journal of Bacteriology 95:2045–2068
    [Google Scholar]
  4. Asakura S., Eguchi G., Iino T. 1968; Unidirectional growth of Salmonella flagella in vitro . Journal of Molecular Biology 35:227–236
    [Google Scholar]
  5. Cohen-Bazire G., London J. 1967; Basal organelles of bacterial flagella. Journal of Bacteriology 94:458–465
    [Google Scholar]
  6. DePamphilis M.L., Adler J. 1971; Fine structure and isolation of the hook-basal body complex of flagella from Escherichia coli and Bacillus subtilis . Journal of Bacteriology 105:384–395
    [Google Scholar]
  7. Iino T. 1969a; Genetics and chemistry of bacterial flagella. Bacteriological Reviews 33:454–475
    [Google Scholar]
  8. Iino T. 1969b; Polarity of flagellar growth in Salmonella. Journal of General Microbiology 56:227–239
    [Google Scholar]
  9. Iino T., Enomoto M. 1966; Genetical studies of non-flagellate mutants of Salmonella . Journal of General Microbiology 43:315–327
    [Google Scholar]
  10. Joys T.M., Stocker B.A.D. 1965; Complementation of nonflagellate Salmonella mutants. Journal of General Microbiology 41:47–55
    [Google Scholar]
  11. Lawn A.M. 1967; Simple immunological labelling method for electron microscopy and its application to the study of filamentous appendages of bacteria. Nature; London: 2141151–1152
    [Google Scholar]
  12. Yamaguchi S., Iino T. 1969; Genetic determination of the antigenic specificity of flagellar protein in Salmonella . Journal of General Microbiology 55:59–74
    [Google Scholar]
  13. Yamaguchi S., Iino T., Horiguchi T., Ohta K. 1972; Genetic analysis of fla and mot cistrons closely linked to H1 in Salmonella abortusequi and its derivatives. Journal of General Microbiology 70:59–76
    [Google Scholar]
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