%0 Journal Article %A Shoesmith, J. G. %A Holland, K. T. %T The Germination of Spores of Clostridium tetani %D 1972 %J Microbiology, %V 70 %N 2 %P 253-261 %@ 1465-2080 %R https://doi.org/10.1099/00221287-70-2-253 %I Microbiology Society, %X SUMMARY The spores of Clostridium tetani will germinate aerobically and anaerobically in complex media such as heart infusion broth or tryptone with yeast extract at 37° and pH 7·5. A synthetic medium as effective as heart infusion broth for germination under both aerobic and anaerobic conditions consists of 1 mm-methionine, 100 mm-lactate, 82 μ m-nicotinamide, 280 mm-Na+ in 40 mm-phosphate buffer, pH 7·5. Anaerobic germination would take place in either methionine with Na+ or lactate and nicotinamide with Na+, but all the germinants were essential aerobically. Heat activation did not occur and thioglycollate increased the rate of germination. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-70-2-253