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The spores of Clostridium tetani will germinate aerobically and anaerobically in complex media such as heart infusion broth or tryptone with yeast extract at 37° and pH 7·5. A synthetic medium as effective as heart infusion broth for germination under both aerobic and anaerobic conditions consists of 1 mm-methionine, 100 mm-lactate, 82 μ m-nicotinamide, 280 mm-Na+ in 40 mm-phosphate buffer, pH 7·5. Anaerobic germination would take place in either methionine with Na+ or lactate and nicotinamide with Na+, but all the germinants were essential aerobically. Heat activation did not occur and thioglycollate increased the rate of germination.
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