SUMMARY: The spores of will germinate aerobically and anaerobically in complex media such as heart infusion broth or tryptone with yeast extract at 37° and pH 7.5. A synthetic medium as effective as heart infusion broth for germination under both aerobic and anaerobic conditions consists of 1 mM-methionine, 100 mM-lactate, 82 μM-nicotinamide, 280 mM-Na in 40 mM-phosphate buffer, pH 7.5. Anaerobic germination would take place in either methionine with Na or lactate and nicotinamide with Na, but all the germinants were essential aerobically. Heat activation did not occur and thioglycollate increased the rate of germination.


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