1887

Abstract

SUMMARY: Carbenicillin resistance in two strains of has been investigated. NCTC 10490, a carbenicillin sensitive strain, produced a β-lactamase when grown in the presence of carbenicillin. This enzyme although active against benzylpenicillin did not hydrolyse carbenicillin. Cell walls of NCTC 10490 habituated to the presence of carbenicillin showed a higher lipid content compared with the walls of the parent strain. Hospitalisolated, carbenicillin-resistant 69/4992 synthesized a β-lactamase which hydrolysed carbenicillin as well as benzylpenicillin. The two types of enzyme were characterized by their substrate profile, sensitivity to enzyme inhibitors and resolution by isoelectric focusing. Enzymes active against carbenicillin were differentiated by isoelectric focusing from those unable to hydrolyse carbenicillin; both types of enzyme were shown to be complex mixtures by this technique.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-70-2-231
1972-04-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/70/2/mic-70-2-231.html?itemId=/content/journal/micro/10.1099/00221287-70-2-231&mimeType=html&fmt=ahah

References

  1. Acred P., Brown D.M., Knudsen E.T., Rolinson G.N., Sutherland R. 1967; New semisynthetic penicillin active against Pseudomonas pyocyanea . Nature; London: 21525–30
    [Google Scholar]
  2. Allen R.J.L. 1940; The estimation of phosphorus. Biochemical Journal 34:858–865
    [Google Scholar]
  3. Bell S.M., Smith D.D. 1969; Resistance of Pseudomonas aeruginosa to carbenicillin. Lancet i:753–754
    [Google Scholar]
  4. Bobo R.A., Eagon R.G. 1968; Lipids of cell walls of Pseudomonas aeruginosa and Brucella abortus . Canadian Journal of Microbiology 14:503–513
    [Google Scholar]
  5. British Pharmacopoeia. 1968 Addendum 1969 London: The Pharmaceutical Press;
    [Google Scholar]
  6. Brown M.R.W., Watkins W.M. 1970; Low magnesium and phospholipid content of cell walls of Pseudomonas aeruginosa resistant to polymyxin. Nature; London: 2271360–1361
    [Google Scholar]
  7. Brumfitt W., Percival A., Leigh D.A. 1967; Clinical and laboratory studies with Carbenicillin. Lancet 1:1289–1293
    [Google Scholar]
  8. Cole M., Sutherland R. 1966; The role of penicillin acylase in the resistance of Gram-negative bacteria to penicillins. Journal of General Microbiology 42:345–356
    [Google Scholar]
  9. Darrell J.H., Waterworth P.M. 1969; Carbenicillin resistance in Pseudomonas aeruginosa from clinical material. British Medical Journal 3:141–143
    [Google Scholar]
  10. Dittmer J.C., Lester R.L. 1964; A simple specific spray for the detection of phospholipids on thin- layer chromatograms. Journal of Lipid Research 5:126
    [Google Scholar]
  11. Dunnick J.K., O’Leary W.M. 1970; Correlation of bacterial lipid composition with antibiotic resistance. Journal of Bacteriology 101:892–900
    [Google Scholar]
  12. Dyke K.G.H. 1969; Penicillinase production and intrinsic resistance to penicillin in methicillin-resistant cultures of Staphylococcus aureus . Journal of Medical Microbiology 2:261–278
    [Google Scholar]
  13. Ellwood D.C., Keleman M.V., Baddiley J. 1963; The glycerol teichoic acid from the walls of Staphylococcus albus ntcc 7944. Biochemical Journal 86:213–225
    [Google Scholar]
  14. Fullbrook P.D., Elson S.W., Slocombe B. 1970; R-factor mediated β-Iactamase in Pseudomonas aeruginosa . Nature; London: 2261054–1056
    [Google Scholar]
  15. Garber N., Friedman J. 1970; β-Lactamase and the resistance of Pseudomonas aeruginosa to various penicillins and cephalosporins. Journal of General Microbiology 64:343–352
    [Google Scholar]
  16. Hancock I.C., Meadow P.M. 1969; The extractable lipids of Pseudomonas aeruginosa . Biochimica et biophysica acta 187:366–379
    [Google Scholar]
  17. Haworth C., Heathcote J.G. 1969; An improved technique for the analysis of amino acids and related compounds on thin-layers of cellulose. I. Qualitative separation. Journal of Chromatography 41:380–385
    [Google Scholar]
  18. Humphreys K.C. 1970; Isoelectric focusing of Trypanosoma brucei subgroup antigens in polyacrylamide gel thin layers. Journal of Chromatography 49:503–510
    [Google Scholar]
  19. Jack G.W., Richmond M.H. 1970; A comparative study of eight distinct β-lactamases synthesized by Gram-negative bacteria. Journal of General Microbiology 61:43–61
    [Google Scholar]
  20. Jones R.J., Lowbury E.J.L. 1967; Prophylaxis and therapy tor Pseudomonas aeruginosa infection with carbenicillin and with gentamicin. British Medical Journal iii:79–82
    [Google Scholar]
  21. Knudsen E.T., Rolinson G.N., Sutherland R. 1967; Carbenicillin: A new semi-synthetic penicillin active against Pseudomonas pyocyanea . British Medical Journal iii:75–78
    [Google Scholar]
  22. Lightbown J.W., De Rossi P. 1965; The identification and assay of mixtures of antibiotics by electrophoresis in agar gel. Analyst 90:89–98
    [Google Scholar]
  23. Lowbury E.J.L., Kidson A., Lilly H.A., Ayliffe G.A.J., Jones R.J. 1969; Sensitivity of Pseudomonas aeruginosa to antibiotics: emergence of strains highly resistant to carbenicillin. Lancet ii:448–452
    [Google Scholar]
  24. Mozingo R., Folkers K. 1949 In The Chemistry of Penicillin p 563 Clark H. T., Johnson J. R., Robinson R. Edited by Princeton, New Jersey: University Press;
    [Google Scholar]
  25. Mukerjee H., Ram J.S. 1964; Paper chromatographic separation of glucosamine and galactosamine. Analytical Biochemistry 8:393–394
    [Google Scholar]
  26. Neu H.C., Swarz H. 1969; Resistance of Escherichia coli and Salmonella typhimurium to carbenicillin. Journal of General Microbiology 58:301–305
    [Google Scholar]
  27. Newson S.W.B. 1969; Carbenicillin-resistant Pseudomonas aeruginosa (correspondence). Lancet ii:1141
    [Google Scholar]
  28. Pollock M.R., Torriani A-M. 1953; The purification and characteristics (physico-chemico) of the penicillinase of Bacillus cereus . Compte rendu des séances de l’Académie des sciences 237:276–278
    [Google Scholar]
  29. Richardson A. E., Spittle C. R., James K. W., Robinson O. P. W. . 1968; Experiences with carbeni- cillin in the treatment of septicaemia and meningitis. Postgraduate Medical Journal 44:844–847
    [Google Scholar]
  30. Rogers J.J., Jeljaszewicz J. 1961; Inhibition of the biosynthesis of cell mucopeptides by the penicillins. A study of a penicillin-sensitive Staphylococcus aureus and a penicillin-resistant Staphylococcus aureus . Biochemical Journal 81:576–584
    [Google Scholar]
  31. Sabath L.D., Jago M., Abraham E.P. 1965; Cephalosporinase and penicillinase activities of a β-lactamase from Pseudomonas aeruginosa . Biochemical Journal 96:739–752
    [Google Scholar]
  32. Schwartz M.A. 1969; Chemical aspects of penicillin allergy. Journal of Pharmaceutical sciences 58:643–661
    [Google Scholar]
  33. Smith C.B., Finland M. 1968; Carbenicillin: activity in vitro and absorption and excretion in normal young men. Applied Microbiology 16:1753–1760
    [Google Scholar]
  34. Smith J.T., Hamilton-Miller J.M.T., Knox R. 1969; Bacterial resistance to penicillins and cephalosporins. Journal of Pharmacy and Pharmacology 21:337–358
    [Google Scholar]
  35. Stephenson J.B.P. 1969; Resistance of Pseudomonas aeruginosa to carbenicillin (correspondence). Lancet i:1098–1099
    [Google Scholar]
  36. Stratford B.C. 1968; The treatment of infections due to Pseudomonas aeruginosa with carbenicillin. Medical Journal of Australia 2:890–895
    [Google Scholar]
  37. Sykes R.B., Richmond M.H. 1970; Intergeneric transfer of a β-lactamase gene between P. aeruginosaand E. coli . Nature; London: 226952–954
    [Google Scholar]
  38. Thomas A.H., Broadbridge R.A. 1970; Electrophoretic separation of penicillins and penicilloic acids. Analyst 95:459–462
    [Google Scholar]
  39. Watanakunakorn C., Phair J.P., Hamburger M. 1970; Increased resistance of Pseudomonas aeruginosa to carbenicillin after reversion from spheroplast to rod form. Infection & Immunity 1:427–430
    [Google Scholar]
  40. Wilkinson S.G. 1968; Studies on the cell walls of Pseudomonas species resistant to ethylenediaminetetra- acetic acid. Journal of General Microbiology 54:195–213
    [Google Scholar]
  41. Wise W.S., Twigg G.H. 1950; Determination of penicillinase activity. Analyst 75:106–108
    [Google Scholar]
  42. Ziminski T., Borowski E. 1966; A new spray reagent replacing sulphuric acid in thin-layer chromatography. Journal of Chromatography 23:480
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-70-2-231
Loading
/content/journal/micro/10.1099/00221287-70-2-231
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error