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Procedures for mutagenesis and conditions for selection by penicillin enrichment in the blue-green alga Anacystis nidulans have been developed. The characterization of 19 mutant strains involving single or serially induced multiple markers was achieved. In addition to drug-resistant and morphological mutants auxotrophic strains requiring phenylalanine, methionine, biotin or acetate together with strains deficient in sulphate and nitrate reduction have been isolated.
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