1887

Abstract

SUMMARY

Root nodules formed by a riboflavin-requiring auxotroph of (TI/D-his-15) on red clover L.) seedlings grown with or without added riboflavin, showed changes in structure or acetylene-reducing activity when compared with effective nodules produced by the parent strain (). Additional riboflavin was essential for the conversion of vegetative bacteria of the mutant strain into functional, N-fixing bacteroids within the nodules. If riboflavin was not added to the plants, a large proportion of the rhizobia in the host cells failed to develop, and the nodules were almost ineffective, reducing acetylene at a very low rate. When riboflavin was added to the plant growth medium, even as late as 8 days after inoculation, most mutant rhizobia survived and continued to develop into normal bacteroids. This requirement for riboflavin was most critical 1 to 3 days after the appearance of the nodules (about 7 to 9 days after inoculation). If the auxotroph was sustained through this period with added riboflavin, the vitamin could then be removed from the plant substrate and the nodules continued to develop effectively. Impairment in the release of rhizobia from infection threads was observed in older nodules formed by TI/-his-15 in the absence of riboflavin, the rhizobia becoming enveloped in large masses of polysaccharide-like material within the host cells.

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/content/journal/micro/10.1099/00221287-70-2-161
1972-04-01
2022-01-28
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References

  1. Appleby C. A. 1967; Electron transport systems of rhizobium japonicum, 1. Haemoprotein P-450, other co-reactive pigments, cytochromes and oxidases in bacteroids from N2-fixing root nodules. Biochimica et biophysica acta 172:71–87
    [Google Scholar]
  2. Appleby C. A., Bergensen F. J. 1958; . Cytochromes of Rhizobium Nature; London: 1821174
    [Google Scholar]
  3. Bergersen F. J. 1955; The cytology of bacteroids from root nodules of subterranean clover (Trifolium subterraneum L.). Journal of General microbiology 13:411–419
    [Google Scholar]
  4. Bergersen F. J. 1970; The quantitative relationship between nitrogen fixation and the acetylene-reduction assay. Australian Journal of Biological Sciences 23:1015–1025
    [Google Scholar]
  5. Bergersen F. J., Briggs M. J. 1958; Studies on the bacterial component of soybean root nodules: cytology and organization in the host tissue. Journal of General Microbiology 19:482–490
    [Google Scholar]
  6. Brenchley W. E., Thornton H. G. 1925; The relation between the development, structure and functioning of the nodules on VICIA FABA, as influenced by the presence or absence of boron in the nutrient medium. Proceedings of the Royal Society Of London B 98:373–399
    [Google Scholar]
  7. Dart P. J., Mercer F. V. 1963; Development of the bacteroid in the root nodules of barrel medic (Medicago tribuloides desr.) and subterraneum clover (trifolium subterraneum l.). Archiv für mikro-biologie 46:382–401
    [Google Scholar]
  8. Dart P. J., Mercer F. V. 1964; Fine structure changes in the development of nodules of trifolium subterraneum L., and medicago tribuloides desr. archiv für mikrobiologie 49:209–235
    [Google Scholar]
  9. Dixon R. O. D. 1967; The origin of the membrane envelope surrounding the bacteria and bacteroids and the presence of glycogen in clover root nodules. Archiv für mikrobiologie 56:156–166
    [Google Scholar]
  10. Fahraeus G., Ljunggren H. 1968; Pre-infection phases of the legume symbiosis The Ecology of Soil Bacteria . An International Symposium pp 396–421 Liverpool: University Press;
    [Google Scholar]
  11. Fottrell P. F. 1966; Dehydrogenase isoenzymes from legume root nodules. Nature; London: 210198–199
    [Google Scholar]
  12. Goodchild D. J., Bergersen F. J. 1966; Electron microscopy of the infection and subsequent development of soybean nodule cells. Journal of Bacteriology 92:204–213
    [Google Scholar]
  13. Huber J. D., Parker F., Odland G. F. 1968; A basic fuchsin and alkalinized methylene blue rapid stain for epoxy-embedded tissue. Stain Technology 43:83–87
    [Google Scholar]
  14. Mosse B. 1964; Electron microscope studies of nodule development in some clover species. Journal of General Microbiology 36:49–66
    [Google Scholar]
  15. Moustafa E., Greenwood R. M. 1967; Esterase and phosphatase isoenzymes in rhizobial bacteroids in relation to strain effectiveness and proportion of nodule tissue in Lotus. New Zealand Journal Of Science 10:548–555
    [Google Scholar]
  16. Roughley R. J., Dart P. J., Nutman P. S. 1970; The infection of Trifolium Subterraneum root-hairs by Rhizobium Trifolii. Journal Of Experimental Botany 21:186–194
    [Google Scholar]
  17. Schwinghamer E. A. 1960; Studies on induced variation in the rhizobia. 1. Defined media and nodulation test techniques. Journal Of Applied Microbiology 8:349–352
    [Google Scholar]
  18. Schwinghamer E. A. 1967; Loss of effectiveness and infectivity in mutants of Rhizobium resistant to metabolic inhibitors. Canadian Journal of Microbiology 14:355–367
    [Google Scholar]
  19. Schwinghamer E. A. 1969; Mutation to auxotrophy and prototrophy as related to symbiotic effectiveness in Rhizobium Leguminosarum and R. Trifolii. Canadian Journal of Microbiology 15:611–622
    [Google Scholar]
  20. Schwinghamer E. A. 1970; Requirement for riboflavin for effective symbiosis on clover by an auxotrophic mutant strain of Rhizobium Trifolii. Australian Journal of Biological Sciences 23:1187–1196
    [Google Scholar]
  21. Shemakhanova N. M., Bun’ko I. P. 1969; Activity criteria for the nodule bacteria of beans. Mikrobiologiya 38:1070–1074
    [Google Scholar]
  22. Thornton H. G. 1930; The influence of the host plant in inducing parasitism in lucerne and clover nodules. Proceedings of The Royal Society Of London B106:110–122
    [Google Scholar]
  23. Tuzimura K. 1950; Lactoflavin in the root-nodules of leguminous plants. Journal of The Agricultural Chemistry Society Of Japan 24:97–100
    [Google Scholar]
  24. Williams C. H., Twine J. R. 1967; Determination of nitrogen, sulphur, phosphorus, potassium, sodium, calcium, and magnesium in plant material by automatic analysis. Technical Papers, Division Of Plant Industry, C.S.I.R.O. Australia:24
    [Google Scholar]
  25. Wong P., Evans H. J., Klucas R. P., Russell S. 1972; Investigations into the pathway of electron transport to the nitrogenase from nodule bacteroids. Plant and Soi in the press
    [Google Scholar]
  26. Yao Phaik Y., Vincent J. M. 1968; Host specificity in the root-hair ‘curling factor’ of RHIZOBIUM spp. Australian Journal of Biological Sciences 22:413–423
    [Google Scholar]
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