@article{mbs:/content/journal/micro/10.1099/00221287-70-1-59, author = "Yamaguchi, S. and Iino, T. and Horiguchi, T. and Ohta, K.", title = "Genetic Analysis of fla and mot Cistrons Closely Linked to H1 in Salmonella abortusequi and its Derivatives", journal= "Microbiology", year = "1972", volume = "70", number = "1", pages = "59-75", doi = "https://doi.org/10.1099/00221287-70-1-59", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-70-1-59", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: Spontaneous non-flagellate and paralysed mutants were isolated from a phase-1 stable strain of Salmonella abortusequi and its H1-replaced derivatives. Trail production in transduction between these mutants resulting from complementation was tested. Fifteen cistrons were recognized: fla A, flaB, flaC, flaD, flaE, flaF, flaK, JlaL,flaM, motA, motB, and H1 (ah 1) which were already known, and three new ones, flaN,flaP, and flaQ. All, except flaF and fiaM, were co-transducible with H1. Fla A mutants fell into three subgroups, flaAI, flaAll, and flaAIII. Some paralysed mutants belonged to the same group as non-flagellate flaAII mutants, and their average numbers of flagella per bacterium varied with the strain from o to nearly the same as wild-type. Several single site flaAII and flaN mutants showed non-reciprocal complementation; as recipients in complementation tests they gave shorter and often fewer trails than when they were donors. Deletion mutants covering most or all of the flaAII or N cistrons showed normal complementation, suggesting the shorter trails with single site mutants to be the result of incomplete complementation by defective products of the mutated gene. Fla and mot mutations closely linked to H1 were marked with deletion mutants and their sequences determined in three-factor reciprocal crosses. Two gene clusters were found, and the sequence of cistrons in these clusters was -flaD-flaB-flaQ-flaP-flaN-flaAlII-flaAII-flaAI-H1(ah1-flaL-and-(flaE,flaK)-motA-motB-flaC-flaM-. All the paralysed flaAII mutations were located at one end of flaAII. ", }