Summary: Spontaneous non-flagellate and paralysed mutants were isolated from a phase-1 stable strain of and its -replaced derivatives. Trail production in transduction between these mutants resulting from complementation was tested. Fifteen cistrons were recognized: , and ( ) which were already known, and three new ones, , and All, except and , were co-transducible with . mutants fell into three subgroups, , and Some paralysed mutants belonged to the same group as non-flagellate mutants, and their average numbers of flagella per bacterium varied with the strain from o to nearly the same as wild-type. Several single site and mutants showed non-reciprocal complementation; as recipients in complementation tests they gave shorter and often fewer trails than when they were donors. Deletion mutants covering most or all of the or cistrons showed normal complementation, suggesting the shorter trails with single site mutants to be the result of incomplete complementation by defective products of the mutated gene.

and mutations closely linked to were marked with deletion mutants and their sequences determined in three-factor reciprocal crosses. Two gene clusters were found, and the sequence of cistrons in these clusters was- ( )--and-(-. All the paralysed mutations were located at one end of


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