1887

Abstract

SUMMARY: Spontaneous non-flagellate and paralysed mutants were isolated from a phase-1 stable strain of and its -replaced derivatives. Trail production in transduction between these mutants resulting from complementation was tested. Fifteen cistrons were recognized: and ) which were already known, and three new and All, except and were co-transducible with mutants fell into three subgroups, and Some paralysed mutants belonged to the same group as non-flagellate mutants, and their average numbers of flagella per bacterium varied with the strain from o to nearly the same as wild-type. Several single site and mutants showed non-reciprocal complementation; as recipients in complementation tests they gave shorter and often fewer trails than when they were donors. Deletion mutants covering most or all of cistrons showed normal complementation, suggesting the shorter trails with single site mutants to be the result of incomplete complementation by defective products of the mutated gene.

and mutations closely linked to were marked with deletion mutants and their sequences determined in three-factor reciprocal crosses. Two gene clusters were found, and the sequence of cistrons in these clusters was -and-( All the paralysed mutations were located at one end of

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-70-1-59
1972-04-01
2022-01-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/70/1/mic-70-1-59.html?itemId=/content/journal/micro/10.1099/00221287-70-1-59&mimeType=html&fmt=ahah

References

  1. Abram D., Vatter A. E., Koffler H. 1966; Attachment and structural features OF flagella OF certain bacilli. Journal of Bacteriology 91:2045–2068
    [Google Scholar]
  2. Adams M. H. 1959 Bacteriophages New York: Interscience Publishers;
    [Google Scholar]
  3. Armstrong J. B., Adler J. 1967; Genetics of motility in Escherichia coli: complementation of paralysed mutants. Genetics 56:363–373
    [Google Scholar]
  4. Cohen-Bazire G., London J. 1967; Basal organelles of bacterial flagella. Journal of Bacteriology 94:458–465
    [Google Scholar]
  5. Edwards P. R., Ewing W. H. 1955 Identification of Enterobacteriaceae. Minneapolis: Burgess Publ Co;
    [Google Scholar]
  6. Enomoto M. 1966a; Genetic studies of paralysed mutants in Salmonella. 1. Genetic fine structure of the mot loci in Salmonella typhimurium. Genetics 54:715–726
    [Google Scholar]
  7. Enomoto M. 1966b; Genetic studies of paralysed mutants in Salmonella. 2. Mapping of three mot loci by linkage analysis. Genetics 54:1069–1076
    [Google Scholar]
  8. Enomoto M. 1967; Composition of chromosome fragments participating in phage P22-mediated transduction of Salmonella typhimurium. Virology 33:474–482
    [Google Scholar]
  9. Enomoto M., Iino T. 1963; Colonial dimorphism in nonmotile Salmonella. Journal of Bacteriology 86:473–477
    [Google Scholar]
  10. Glauert A.M, Kerridge D, Horne R. W. 1963; The fine structure and mode of attachment of the sheathed flagellum of Vibrio metchinikovii. Journal of Cell Biology 18:327–337
    [Google Scholar]
  11. Hoeniger J. F. M. 1965; Development of flagella by Proteus mirabilis. Journal of General Microbiology 40:29–42
    [Google Scholar]
  12. Iino T. 1961; Genetic analysis of O-H variation in Salmonella. Japanese Journal of Genetics 36:268–275
    [Google Scholar]
  13. Iino T. 1962; Phase specific regulation of the flagellin genes (H1 and H2) in Salmonella. Annual Report of National Institute of Genetics Japan: 1372–73
    [Google Scholar]
  14. Iino T. 1969; Genetics and chemistry of bacterial flagella. Bacteriological Reviews 33:454–475
    [Google Scholar]
  15. Iino T., Enomoto M. 1966; Genetical studies of non-flagellate mutants of Salmonella. Journal of General Microbiology 43:315–327
    [Google Scholar]
  16. Iino T., Lederberg J. 1964; Genetics of Salmonella. In The World Problem of Salmonellosis pp. The Hague: Dr W. Junk, Publishers;
    [Google Scholar]
  17. Joys T. M. 1968; The structure of flagella and the genetic control of flagellation in Eubacteriales. A review. Antonie van Leeuwenhoek 34:205–225
    [Google Scholar]
  18. Joys T. M., Stocker B. A. D. 1965; Complementation of non-flagellate Salmonella mutants. Journal of General Microbiology 41:47–55
    [Google Scholar]
  19. Joys T. M., Stocker B. A. D. 1966; Isolation and serological analysis of mutant forms of flagellar antigen i of Salmonella typhimurium. Journal of General Microbiology 44:121–138
    [Google Scholar]
  20. Joys T. M., Stocker B. A. D. 1969; Recombination in H1, the gene determining the flagellar antigen-i of Salmonella typhimurium; mapping of H1 and fla mutations. Journal of General Microbiology 58:267–275
    [Google Scholar]
  21. Kerridge D., Horne R. W., Glauert A. M. 1962; Structural components of flagella from Sdlmonella typhimurium. Journal of Molecular Biology 4:227–238
    [Google Scholar]
  22. Lederberg J. 1956; Linear inheritance in transductional clones. Genetics 41:845–871
    [Google Scholar]
  23. Leifson E. 1951; Staining, shape, and arrangement OF bacterial flagella. Journal of Bacteriology 62:377–389
    [Google Scholar]
  24. Lowy J. 1965; Structure of the proximal ends of bacterial flagella. Journal of Molecular Biology 14:297–299
    [Google Scholar]
  25. Mäkelä P. H. 1964; Genetic homologies between flagellar antigens of Escherichia coli and Salmonella abony. Journal of General Microbiology 35:503–510
    [Google Scholar]
  26. Meynell E. W. 1961; A phage, 𝜙 χ, which attacks motile bacteria. Journal of General Microbiology 25:253–290
    [Google Scholar]
  27. Sasaki I. 1962; χ-phage resistance in Salmonella having g-group antigen (Text in Japanese, English summary and tables). Virus 12:168–176
    [Google Scholar]
  28. Sanderson K. E. 1970; Current linkage map of Salmonella typhimurium. Bacteriological Reviews 34:176–193
    [Google Scholar]
  29. Smith S. M., Stocker B. A. D. 1962; Colicinogeny and recombination. British Medical Bulletin 18:46–51
    [Google Scholar]
  30. Stocker B. A. D. 1956; Abortive transduction of motility in Salmonella; a non-replicated gene transmitted through many generations to a single descendant. Journal of General Microbiology 15:575–598
    [Google Scholar]
  31. Stocker B. A. D., Mcdonough M. W., Ambler R. P. 1961; A gene determining presence or absence of ε-N-methyllysine in Salmonella flagellar protein. Nature; London: 189556–558
    [Google Scholar]
  32. Yamaguchi S. 1968; Sensitivity of the g-complex antigenic Salmonella strains to M8, a host-range mutant of bacteriophage χ. Journal of General Virology 2:187–190
    [Google Scholar]
  33. Yamaguchi S., Iino T. 1969; Genetic determination of the antigenic specificity of flagellar protein in Salmonella. Journal of General Microbiology 55:59–74
    [Google Scholar]
  34. Zipser D., Perrin D. 1963; Complementation on ribosomes. Cold Spring Harbor Symposia on Quantitative Biology 28:533–537
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-70-1-59
Loading
/content/journal/micro/10.1099/00221287-70-1-59
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error