Distribution of Wall-bound Invertase during the Asexual Life-cycle of Free

Abstract

SUMMARY

The association of invertase with Neurospora wall was studied biochemically in six cytologically distinct stages of the Neurospora asexual life-cycle: (i) conidia, (ii) mid-log phase [10 h old], (iii) post-log phase hyphae [18 h old], (iv) aerial mycelium [4 days old], (v) submerged mycelium [4 days old], and (vi) mycelium [2 days old] induced to form conidiophores. The percentages of total invertase activity found in the wall fraction were 8, 18, 23, 8, 21 and 17, respectively. The specific activities of the wall fraction were 3, 85, 120, 40, 24, and 74 units of invertase activity per milligram protein, respectively. Histochemical localization of invertase with an indirect immunofluorescent technique also showed that conidiophores had strong activity in their walls as well as in the cytoplasm and that vacuoles were devoid of any activity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-70-1-23
1972-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/70/1/mic-70-1-23.html?itemId=/content/journal/micro/10.1099/00221287-70-1-23&mimeType=html&fmt=ahah

References

  1. Beadle G. W., Tatum E. L. 1945; Neurospora. II. Methods of producing and detecting mutations concerned with nutritional requirements. American Journal of Botany 32:678–686
    [Google Scholar]
  2. Beteta P., Gascon S. 1971; Localization of invertase in yeast vacuoles. FEBS Letters 13:297–300
    [Google Scholar]
  3. Campbell D. H., Garvey J. S., Cremer N. E., Sussdorf D. H. 1963 Methods in Immunology pp 12–23143–149 New York: W. A. Benjamin;
    [Google Scholar]
  4. Chang P. L. Y., Trevithick J. R. 1972; Release of wall-bound invertase and trehalase in Neurospora crassa by hydrolytic enzymes. Journal of General Microbiology 70:13–22
    [Google Scholar]
  5. Chung P. L. Y., Trevithick J. R. 1970; Biochemical and histochemical localization of invertase in Neurospora crassa during conidial germination and hyphal growth. Journal of Bacteriology 102:423–429
    [Google Scholar]
  6. Eberhart B. M. 1961; Exogenous enzymes of Neurospora conidia and mycelia. Journal of Cellular and Comparative Physiology 58:11–16
    [Google Scholar]
  7. Hill E. P., Sussman A. S. 1964; Development of trehalase and invertase activity in Neurospora . Journal of Bacteriology 88:1556–1566
    [Google Scholar]
  8. Horikoshi K., Ikeda Y. 1965; Studies on the spore coats of Aspergillus oryzae. II. Conidia coat-bound β-glucosidase. Biochimica et biophysica acta 101:352–357
    [Google Scholar]
  9. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  10. Mahadevan P. R., Mahadkar U. R. 1970; Role of enzymes in growth and morphology of Neurospora crassa: cell-wall-bound enzymes and their possible role in branching. Journal of Bacteriology 101:941–947
    [Google Scholar]
  11. Metzenberg R. L. 1962; A gene affecting the repression of invertase and trehalase in Neurospora. Archives of Biochemistry and Biophysics 96:468–474
    [Google Scholar]
  12. Metzenberg R. L. 1963a; The purification and properties of invertase of Neurospora . Archives of Bio-chemistry and Biophysics 100:503–511
    [Google Scholar]
  13. Metzenberg R. L. 1963b; The localization of a β-fructofuranosidase in Neurospora. Biochimica et biophysica acta 77:455–465
    [Google Scholar]
  14. Metzenberg R. L. 1964; Enzymically active subunits of Neurospora invertase. Biochimica et biophysica acta 89:291–302
    [Google Scholar]
  15. Nagasaki S. 1968; Cytological and physiological studies on phosphatases in developing cultures of Aspergillus niger . Journal of General and Applied Microbiology Tokyo: 14263–277
    [Google Scholar]
  16. Nairn R. O. 1969 Fluorescent Protein Tracing, 3rd edn. pp 129–130147–148 Edinburgh: E. and S. Livingstone;
    [Google Scholar]
  17. Palade G. E., Siekevitz P., Cro L. G. 1962; Structure, chemistry, and function of the pancreatic exocrine cell. In The Exocrine Pancreas pp 23–55 De Reuck A. V. S., Cameron M. P. Edited by Ciba Foundation Symposium. London:: J. and A. Churchill;
    [Google Scholar]
  18. Scott W. A., Metzenberg R. L. 1970; Location of aryl sulfatase in conidia and young mycelia of Neurospora crassa . Journal of Bacteriology 104:1254–1265
    [Google Scholar]
  19. Shne G. J. 1968; Enzyme activities during the asexual cycle of Neurospora crassa. II. NAD-& NADP-dependent glutamic dehydrogenases and nicotinamide adenine dinucleotidase. Journal of Cell Biology 37:81–88
    [Google Scholar]
  20. Stine G. J. 1969; Enzyme activities during the asexual cycle of Neurospora crassa. III. Nicotinamide adenosine diphosphate glycohydrolase. Canadian Journal of Microbiology 15:1249–1254
    [Google Scholar]
  21. Stine G. J., Clark A. M. 1967; Synchronous production of conidiophores and conidia of Neurospora crassa . Canadian Journal of Microbiology 13:447–453
    [Google Scholar]
  22. Weiss B. 1963; An electron microscope and biochemical study of Neurospora crassa during development. Journal of General Microbiology 39:85–94
    [Google Scholar]
  23. Zalokar M. 1959; Enzyme activity and cell differentiation in Neurospora. American Journal of Botany 46:555–559
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-70-1-23
Loading
/content/journal/micro/10.1099/00221287-70-1-23
Loading

Data & Media loading...

Most cited Most Cited RSS feed