1887

Abstract

Summary: The production of a rennin-like milk-clotting enzyme by 805 was investigated. Corn-steep water was the best medium for the production of the enzyme. Dephytinization of corn-steep water lowered production of the enzyme. The extent of mycelial sporulation was correlated with the milk-clotting activity. Precipitation with ammonium sulphate and ethanol was unsuitable, but acetone produced active fractions. The enzyme action was optimal at 60° and at pH 6·0 while the stability of the enzyme was maximal at pH 5·37. The enzyme exhibited feeble proteolytic activity.

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/content/journal/micro/10.1099/00221287-70-1-151
1972-04-01
2021-10-24
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