1887

Abstract

SUMMARY: A strain of yeast called 2n is described which behaved genetically as a diploid, but had approximately 18 % less DNA than a normal diploid. It produced apparently normal haploid ascospore cultures, which had approximately 20 % less DNA than normal haploids. The growth of 2n from low yeast concentrations was normal, but at high concentrations it was abnormal; viability was not affected. The timing of DNA synthesis in a synchronously dividing culture was in the usual position in the growth cycle for yeast. DNA was estimated by the diphenyl-amine reaction; there was no special effect on this reaction, peculiar to 2n and its ascospore cultures. DNA values were confirmed by measurements. The yeast 2n was more sensitive to both gamma and u.v. radiations than were normal diploids and there was some indication that defective repair processes were involved.

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/content/journal/micro/10.1099/00221287-70-1-129
1972-04-01
2024-04-25
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References

  1. Beam C. A., Mortimer R. K., Wolfe R. G., Tobias C. A. 1954; The relation of radioresistance to budding in Saccharomyces cerevisiae. Archives of Biochemistry and Biophysics 49:110–122
    [Google Scholar]
  2. Bevan E. A., Costello W. P. 1964; The preparation and use of an enzyme which breaks open yeast asci. Microbial Genetics Bulletin 21:5
    [Google Scholar]
  3. Boivin A., Vendrely R., Vendrely C. 1948; L’acide désoxyribonucléique du noyau cellulaire, dépositaire des caractères héréditaires; arguments d’ordre analytique. Comptes rendus hebdomadaire des seances de I’Académie des sciences 226:1061–1063
    [Google Scholar]
  4. Britten R. J., Davidson E. H. 1969; Gene regulation for higher cells: a theory. Science; New York: 165349–357
    [Google Scholar]
  5. Britten R. J., Kohne D. E. 1968; Repeated sequences in DNA. Science; New York: 161529–533
    [Google Scholar]
  6. Burton K. 1956; A study of the conditions and the mechanism of the diphenylamine reaction for the colorimetric estimation of DNA. Biochemical Journal 62:315–323
    [Google Scholar]
  7. Burton K. 1968; Determination of DNA concentration with diphenylamine. In Methods in Enzymology XII pp. 163–166 Grossman L., Moldave K. Edited by New York & London: Academic Press;
    [Google Scholar]
  8. Callan H. G. 1967; The organization of genetic units in chromosomes. Journal of Cellular Science 2:1–7
    [Google Scholar]
  9. Cox B. S., Bevan E. A. 1962; Aneuploidy in yeast. New Phytologist 61:342–355
    [Google Scholar]
  10. Hatchard C. G., Parker C. A. 1956; A new sensitive chemical actinometer. II. Potassium ferrioxalate as a standard chemical actinometer. Proceedings of the Royal Society A 235:518–536
    [Google Scholar]
  11. Haynes R. H. 1964; Role of DNA repair mechanisms in microbial inactivation and recovery phenomena. Photochemistry and Photobiology 3:429–450
    [Google Scholar]
  12. Haynes R. H. 1966; The interpretation of microbial inactivation and recovery phenomena. Radiation Research Suppl 6:1–45
    [Google Scholar]
  13. Heagy F. C., Roper J. A. 1952; Deoxyribonucleic acid content of haploid and diploid Aspergillusconidia. Nature; London: 170713–714
    [Google Scholar]
  14. Holliday R. 1961; The genetics of Ustilago maydis. Genetical Research 2:204–230
    [Google Scholar]
  15. Hurst D. D., Fogel S. 1964; Mitotic recombination and heteroallelic repair in Saccharomyces cerevisiae. Genetics 50:435–458
    [Google Scholar]
  16. Jones R. N., Rees H. 1968; Nuclear DNA variation in Allium. Heredity 23:591–605
    [Google Scholar]
  17. Lea D. E., Haines R. B., Coulson C. A. 1936; The mechanism of the bactericidal action of radioactive radiations. Proceedings of the Royal Society B 120:47–66
    [Google Scholar]
  18. Levinthal N., Fogel S., Hurst D. D. 1962; Genetic and biochemical analysis of purine biosynthetic pathway in Saccharomyces. Genetics 47: abstr 967
    [Google Scholar]
  19. Mirsky A. E., Ris H. 1949; Variable and constant components of chromosomes. Nature; London: 163666–667
    [Google Scholar]
  20. Mirsky A. E., Ris H. 1951; The DNA content of animal cells and its evolutionary significance. Journal of General Physiology 34:451–462
    [Google Scholar]
  21. Mortimer R. K., Hawthorne D. C. 1966; Genetic mapping in Saccharomyces. Genetics 53:165–173
    [Google Scholar]
  22. Moustacchi E., Williamson D. H. 1966; Physiological variations in satellite components of yeast DNA detected by density gradient centrifugation. Biochemical and Biophysical Research Communication 23:56–61
    [Google Scholar]
  23. Ogur M., Minchler S., Lindegren G., Lindegren C. C. 1952; The nucleic acids in a polyploid series of Saccharomyces. Archives of Biochemistry and Biophysics 40:175–184
    [Google Scholar]
  24. Ogur M., Rosen G. 1949; The nucleic acids of plant tissues. I. The extraction and estimation of deoxypentose nucleic acid and pentose nucleic acid. Archives of Biochemistry and Biophysics 25:262–276
    [Google Scholar]
  25. Pollister A. W., Swift H., Alfert M. 1951; The desoxypentose nucleic acid content of animal nuclei. Experimental Cell Research Suppl1 2:59–73
    [Google Scholar]
  26. Stebbins G. L. 1966; Chromosome variation and evolution. Science; New York: 1521463–1469
    [Google Scholar]
  27. Walker P. M. B. 1971; Origin of satellite DNA. Nature; London: 229306–308
    [Google Scholar]
  28. Williamson D. H. 1965; The timing of DNA synthesis in the cell cycle of Saccharomyces cerevisiae. Journal of Cell Biology 25:517–528
    [Google Scholar]
  29. Williamson D. H., Scopes A. W. 1960; The behaviour of nucleic acids in synchronously dividing cultures of Saccharomyces cerevisiae. Experimental Cell Research 20:338–349
    [Google Scholar]
  30. Williamson D. H., Scopes A. W. 1962; A rapid method for synchronising division in Saccharomyces cerevisiae. Nature; London: 193256–257
    [Google Scholar]
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