1887

Abstract

SUMMARY: A strain of yeast called 2n is described which behaved genetically as a diploid, but had approximately 18 % less DNA than a normal diploid. It produced apparently normal haploid ascospore cultures, which had approximately 20 % less DNA than normal haploids. The growth of 2n from low yeast concentrations was normal, but at high concentrations it was abnormal; viability was not affected. The timing of DNA synthesis in a synchronously dividing culture was in the usual position in the growth cycle for yeast. DNA was estimated by the diphenyl-amine reaction; there was no special effect on this reaction, peculiar to 2n and its ascospore cultures. DNA values were confirmed by measurements. The yeast 2n was more sensitive to both gamma and u.v. radiations than were normal diploids and there was some indication that defective repair processes were involved.

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/content/journal/micro/10.1099/00221287-70-1-129
1972-04-01
2022-08-08
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