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The Escherichia coli k, b and phage P1 host specificity genes (hsp) were introduced into Salmonella typhimurium by means of E. coli Hfr × S. typhimurium F− crosses. The three systems were found to govern modification and restriction of phages P22 and L. The restriction coefficients were comparable to that of λ for the PI system and were lower (about 100) for the k and b systems. Phage grown on hspK+ and hspB+ recombinants was restricted by S. typhimurium independently of the restriction governed by the lt system. This observation suggested that S. typhimurium possesses a previously undetected hsp locus allelic to those of E. coli k and b.
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