1887

Abstract

Two serologically unrelated strains of nodule bacteria produced two different polysaccharides, only one of which precipitated with antiserum to its parent bacterium. Both polysaccharides interfered with the multiplication of two bacteriophages in liquid cultures of the two bacterial strains, each of which was susceptible to only one of the two bacteriophages. One polysaccharide was slightly more effective than the other in interfering with multiplication of both bacteriophages: one phage was much more susceptible than the other to the interfering action of both polysaccharides. Crystallized pancreatic ribonuclease interfered with multiplication of bacteriophages much more strongly than did the polysaccharides. Neither the polysaccharides nor ribonuclease destroyed the phage particles.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-7-3-4-340
1952-11-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/7/3-4/mic-7-3-4-340.html?itemId=/content/journal/micro/10.1099/00221287-7-3-4-340&mimeType=html&fmt=ahah

References

  1. Ashenburg N. J., Sandholzer L. A., Scherp H. W., Berry G. P. 1940; The influence of bacterial and non-bacterial polysaccharides upon bacteriophagy. J. Bact 39:71
    [Google Scholar]
  2. Bawden F. C., Freeman G. G. 1952; The nature and behaviour of inhibitors produced by Trichothecium roseum Link. J. gen. Microbiol 7:154
    [Google Scholar]
  3. Burnet F. M. 1934; The phage-inactivating agent of bacterial extracts. J. Path. Bact 38:285
    [Google Scholar]
  4. Burnet F. M., Keogh E. V., Lush D. 1937; Immunological reactions of filterable viruses. Aust. J. exp. Biol. med. Sci 15:227
    [Google Scholar]
  5. Cooper E. A., Daker W. D., Stacey M. 1938; Enzyme formation and polysaccharide synthesis by bacteria. III. Polysaccharides produced by ‘nitrogen fixing’ organisms. Biochem. J 32:1752
    [Google Scholar]
  6. Ginsberg H. S., Goebel W. F., Horsfall F. L. 1948; The inhibitory effect of polysaccharide on mumps multiplication. J. exp. Med 87:385
    [Google Scholar]
  7. Gough G. A. C., Burnet F. M. 1934; The chemical nature of the phageinactivating agent in bacterial extracts. J. Path. Bact 38:301
    [Google Scholar]
  8. Green R. M., Wooley D. W. 1947; Inhibition by certain polysaccharides of haemagglutination and multiplication of influenza virus. J. exp. Med 86:55
    [Google Scholar]
  9. Heidelberger M., Kendall F. E., Scherp H. W. 1936; Specific polysaccharides of types I, II and III pneumococcus. J. exp. Med 64:559
    [Google Scholar]
  10. Hopkins E. W., Peterson W. H., Fred E. B. 1931; Glucoronic acid, a constituent of the gum of root nodule bacteria. J. Amer. chem. Soc 53:306
    [Google Scholar]
  11. Horrocks R. H. 1949; Paper partition chromatography of reducing sugars with benzidine as a spraying reagent. Nature; Lond: 164444
    [Google Scholar]
  12. Kassanis B., Kleczkowski A. 1948; The isolation and some properties of a virus-inhibiting protein from Phytolacca esculenta. J. gen. Microbiol 2:143
    [Google Scholar]
  13. Kleczkowski A. 1948; Proteolytic activity of preparations of crystallized ribonuclease. Biochem. J 42:523
    [Google Scholar]
  14. Kleczkowski A., Kleczkowski J. 1951; The ability of single phage particles to form plaques and to multiply in liquid cultures. J. gen. Microbiol 5:346
    [Google Scholar]
  15. Kleczkowski A., Thornton H. G. 1944; A serological study of root nodule bacteria from pea and clover inoculation groups. J. Bact 48:661
    [Google Scholar]
  16. Kunitz M. 1940; Crystalline ribonuclease. J. gen. Physiol 24:15
    [Google Scholar]
  17. Levine P., Frisch A. W. 1933; Specific inhibition of bacteriophage by bacterial extracts. Proc. Soc. exp. Biol., N.Y 30:993
    [Google Scholar]
  18. Loring H. S. 1942; The reversible inactivation of tobacco mosaic virus by crystalline ribonuclease. J. gen. Physiol 25:497
    [Google Scholar]
  19. Miller E. M., Goebel W. F. 1949; Studies on bacteriophage. I. The relationship between the somatic antigens of Shigella sonnei and their susceptibility to bacterial viruses. J. exp. Med 90:255
    [Google Scholar]
  20. Partridge S. M. 1948; Filter paper partition chromatography of sugars. Biochem. J 42:238
    [Google Scholar]
  21. Sciilüchterer E., Stacey M. 1945; The capsular polysaccharide of Rhizobium radicicolum. J. chem. Soc 776:
    [Google Scholar]
  22. Somogyi M. 1945; A new reagent for the determination of sugars. J. biol. Chem 160:61
    [Google Scholar]
  23. Takahashi W. W. 1942; A virus inactivator from yeast. Science 95:586
    [Google Scholar]
  24. Takahashi W. W. 1946; Properties of a virus inactivator from yeast. Science 104:377
    [Google Scholar]
  25. White P. B. 1936; Differential fixation of cholera phages by extracts of V. cholerae. J. Path. Bact 43:591
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-7-3-4-340
Loading
/content/journal/micro/10.1099/00221287-7-3-4-340
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error