SUMMARY: was grown in surface culture on media containing about 0.6 mC./ml. of P as phosphate, and harvested after 2 days. The spore suspension was heated at 60° for 1 1/2 hr. and washed by centrifuging and resuspending in water six times. The fresh suspension contained about 2 x 10 spores/ml., of which 20 % were viable. The radioactivity was about 0°05 mC./ml. of which more than 95 % was carried by the spores. The radioactivity was sufficient for 10 spores to be readily detected and assessed by conventional Geiger-Müller counting methods. These highly radioactive suspensions are particularly useful in studying the respiratory retention of bacterial aerosols.


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