SUMMARY: Wide fluctuations of phosphorus concentration in the cells occur during the cultivation of on aerated media. The concentration of cell phosphorus increases rapidly during the stationary phase and early lag phase to a peak which coincides with the formation of half the first generation; it declines until the end of this generation and increases once again to a second peak, declining subsequently when growth and division rates fall off. Analyses of the cells during cultivation show that the different concentrations of ribonucleic acid largely account for these variations. The association of the initial phosphorus peak with the first generation has been observed during the cultivation of on several liquid media and on CCY medium at different temperatures. The increase in phosphorus concentration during the first generation can be associated quantitatively with the increase in average cell weight.


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