SUMMARY: A high-frequency donor strain of Pasteurella pseudotuberculosis group A was isolated from a strain which had previously accepted an F′lac episome from Escherichia coli. This donor transferred a variety of genetic markers to P. pseudotuberculosis group A recipients. Transfer frequencies of approximately 10−2/donor bacterium were obtained for early markers from membrane matings at 28°; these frequencies were increased to about 2·5 × 10−1/donor at 37°. Recombinant colonies obtained from membrane matings were free from donor and recipient bacteria but some colonies had more than one recombinant class present. The graded frequencies of marker transfer obtained together with recombinant analysis suggest that markers were transferred linearly.
DemerecM., AdelbergE. A., ClarkA. J., HartmanP. E.1968; A proposal for a uniform nomenclature in bacterial genetics. Journal of General Microbiology 50:1–14
DubnauE., StockerB. A. D.1967; Behaviour of three colicine factors and an R (drug-resistance) factor in Hfr crosses in Salmonella typhimurium. Genetical Research 9:283–297
MatneyT. S., AchenbachN. E.1962; A comment on the fertility of F2 donor types of Escherichia colik12. Biochemical and Biophysical Research Communications 9:285–287
MorrisB. C., BurrowsT. W.1968; Gene transfer studies with Pasteurella pseudotuberculosis. International Symposium on Pseudotuberculosis, Paris 1967. Symposia Series - Immunobiological Standardization 9:275–284