SUMMARY: A high-frequency donor strain of group A was isolated from a strain which had previously accepted an episome from This donor transferred a variety of genetic markers to group A recipients. Transfer frequencies of approximately 10/donor bacterium were obtained for early markers from membrane matings at 28°; these frequencies were increased to about 2.5 × 10/donor at 37°. Recombinant colonies obtained from membrane matings were free from donor and recipient bacteria but some colonies had more than one recombinant class present. The graded frequencies of marker transfer obtained together with recombinant analysis suggest that markers were transferred linearly.


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