1887

Abstract

SUMMARY: A high-frequency donor strain of group A was isolated from a strain which had previously accepted an episome from . This donor transferred a variety of genetic markers to group A recipients. Transfer frequencies of approximately 10/donor bacterium were obtained for early markers from membrane matings at 28°; these frequencies were increased to about 2·5 × 10/donor at 37°. Recombinant colonies obtained from membrane matings were free from donor and recipient bacteria but some colonies had more than one recombinant class present. The graded frequencies of marker transfer obtained together with recombinant analysis suggest that markers were transferred linearly.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-69-3-405
1971-12-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/69/3/mic-69-3-405.html?itemId=/content/journal/micro/10.1099/00221287-69-3-405&mimeType=html&fmt=ahah

References

  1. Anderson T. F. 1958; Recombination and segregation in Escherichia coli. Cold Spring Harbour Symposia on Quantitative Biology 23:47–58
    [Google Scholar]
  2. Burrows T. W., Bacon G. A. 1960; V and W antigens in strains of Pasteurella pseudotuberculosis. British Journal of Experimental Pathology 61:38–44
    [Google Scholar]
  3. Davies D. A. L. 1958; The smooth and rough somatic antigens of Pasteurella pseudotuberculosis. Journal of General Microbiology 18:118–128
    [Google Scholar]
  4. Davis B. D. 1948; Isolation of biochemically deficient mutants of bacteria by penicillin. Journal of American Chemical Society 70:4267
    [Google Scholar]
  5. Demerec M., Adelberg E. A., Clark A. J., Hartman P. E. 1968; A proposal for a uniform nomenclature in bacterial genetics. Journal of General Microbiology 50:1–14
    [Google Scholar]
  6. Dubnau E., Stocker B. A. D. 1967; Behaviour of three colicine factors and an R (drug-resistance) factor in Hfr crosses in Salmonella typhimurium. Genetical Research 9:283–297
    [Google Scholar]
  7. Hayes W. 1953; The mechanism of genetic recombination in Escherichia coli. Cold Spring Harbour Symposia on Quantiative Biology 18:75–93
    [Google Scholar]
  8. Hayes W. 1968 The Genetics of Bacteria and Their Viruses, 2nd edn. Oxford and Edinburgh: Blackwell Scientific Publications;
    [Google Scholar]
  9. Holliday R. 1956; A new method for the identification of biochemical mutants of micro-organisms. Nature; London: 178987
    [Google Scholar]
  10. Johnson E. M., Krauskopf B., Baron L. S. 1965; Genetic mapping of Vi and somatic antigenic determinants in Salmonella. Journal of Bacteriology 90:302–308
    [Google Scholar]
  11. Kaudewitz F. 1959; Production of bacterial mutants with nitrous acid. Nature; London: 1831829–1830
    [Google Scholar]
  12. Lawton W. D., Morris B. C., Burrows T. W. 1968; Gene transfer in strains of Pasteurella pseudotuberculosis. Journal of General Microbiology 52:25–34
    [Google Scholar]
  13. Lawton W. D., Stull H. B. 1971; Chromosome mapping of Pasteurella pseudotuberculosis by interrupted mating. Journal of Bacteriology 105:855–863
    [Google Scholar]
  14. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. Journal of Bacteriology 63:399–406
    [Google Scholar]
  15. Lederberg J., Zinder N. 1948; Concentration of biochemical mutants of bacteria with penicillin. Journal of American Chemical Society 70:4267–4268
    [Google Scholar]
  16. Matney T. S., Achenbach N. E. 1962; A comment on the fertility of F2 donor types of Escherichia coli k12. Biochemical and Biophysical Research Communications 9:285–287
    [Google Scholar]
  17. Morris B. C., Burrows T. W. 1968; Gene transfer studies with Pasteurella pseudotuberculosis. International Symposium on Pseudotuberculosis, Paris 1967. Symposia Series - Immunobiological Standardization 9:275–284
    [Google Scholar]
  18. Sanderson K. E., Demerec M. 1965; The linkage map of Salmonella typhimurium. Genetics 51:897–913
    [Google Scholar]
  19. Sasaki I., Bertani G. 1965; Growth abnormalities in Hfr derivatives of Escherichia coli strain c. Journal of General Microbiology 40:365–376
    [Google Scholar]
  20. Stamp Lord. 1947; The preservation of bacteria by drying. Journal of General Microbiology 1:251–265
    [Google Scholar]
  21. Thal E. 1954 Untersuchungen über Pasteurellapseudotuberculosis Lund, Sweden: Berlingska Boktryckeriet;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-69-3-405
Loading
/content/journal/micro/10.1099/00221287-69-3-405
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error