RT Journal Article SR Electronic(1) A1 Maclennan, D. G. A1 Ousby, J. C. A1 Vasey, R. B. A1 Cotton, N. T.YR 1971 T1 The Influence of Dissolved Oxygen on Pseudomonasam1 Grown on Methanol in Continuous Culture JF Microbiology, VO 69 IS 3 SP 395 OP 404 DO https://doi.org/10.1099/00221287-69-3-395 PB Microbiology Society, SN 1465-2080, AB SUMMARY: Methanol-limited cultures of Pseudomonas ami were grown in chemostats (D, 0·1 h.−1; methanol 10 g./l.) at dissolved oxygen tensions between 1 and 690 mm. Hg. Decreasing the dissolved oxygen tension from no to 28·5 mm. Hg did not affect either respiration or carbon balance, but lowering it further caused a progressive increase in respiration which was maximal at 3 mm. Hg (the lowest dissolved oxygen tension at which a steady state could be maintained at the fixed dilution rate). Increasing the dissolved oxygen tension from 97 to 560 mm. Hg increased production of CO2 and decreased yield; this effect was particularly evident between 420 and 560 mm. Hg. Yield varied little between 560 and 690 mm. Hg but CO2 output decreased and exocellular products increased in concentration indicating a metabolic change. The possible mechanisms involved in metabolic regulation are discussed; it is concluded that dissolved oxygen tensions between 3 mm. and 690 mm. Hg exert a continuous quantitative control over respiration and metabolism of growing organisms. A stoichiometric equation is derived for the growth of Pseudomonas am i on methanol., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-69-3-395