1887

Abstract

SUMMARY: Methanol-limited cultures of were grown in chemostats ( 0·1 h.; methanol 10 g./l.) at dissolved oxygen tensions between 1 and 690 mm. Hg. Decreasing the dissolved oxygen tension from no to 28·5 mm. Hg did not affect either respiration or carbon balance, but lowering it further caused a progressive in respiration which was maximal at 3 mm. Hg (the lowest dissolved oxygen tension at which a steady state could be maintained at the fixed dilution rate). Increasing the dissolved oxygen tension from 97 to 560 mm. Hg increased production of CO and decreased yield; this effect was particularly evident between 420 and 560 mm. Hg. Yield varied little between 560 and 690 mm. Hg but COoutput decreased and exocellular products increased in concentration indicating a metabolic change. The possible mechanisms involved in metabolic regulation are discussed; it is concluded that dissolved oxygen tensions between 3 mm. and 690 mm. Hg exert a continuous quantitative control over respiration and metabolism of growing organisms. A stoichiometric equation is derived for the growth of on methanol.

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/content/journal/micro/10.1099/00221287-69-3-395
1971-12-01
2021-10-23
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