1887

Abstract

SUMMARY: Both laboratory-cultured bacteria and the bacteroid forms of (strain wu8) isolated from serradella root nodules synthesize haem from [C]--aminolaevulinic acid (ALA) and release the product into the incubation medium. Laevulinic acid, an inhibitor of haem synthesis in other organisms, effectively inhibits this synthesis in When compared on the basis of fresh weight, protein or numbers, the laboratory-grown bacteria are more active in the incorporation of [C]-ALA than are the bacteroid forms, either when intact or when disrupted by sonication. A synergistic effect between cytoplasmic (plant) and particulate (bacteroid) extracts from serradella nodules is observed which is greater than that for soybean nodules. It is proposed that the haem synthesis for leghaemoglobin in serradella root nodules is a co-operative effort between plant and bacteroid.

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/content/journal/micro/10.1099/00221287-69-3-385
1971-12-01
2024-11-13
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References

  1. Adler A. D., George P. 1965; Determination of iron in heme compounds. I. Hemin. Analytical Biochemistry II:159–163
    [Google Scholar]
  2. Appleby C. A. 1969a; Electron transport systems of Rhizobium japonicum, I. Haemoprotein P-450, other CO-reactive pigments, cytochromes and oxidases in bacteroids from N2-fixing root nodules. Biochimica et biophysica acta 172:71–87
    [Google Scholar]
  3. Appleby C. A. 1969b; Electron transport systems of Rhizobium japonicum. II. Rhizobium haemoglobin, cytochromes and oxidases in free-living (cultured) cells. Biochimica et biophysica acta 172:88–105
    [Google Scholar]
  4. Beale S. I. 1970; The biosynthesis of δ-aminolaevulinic acid in Chlorella. Plant Physiology 45:504–506
    [Google Scholar]
  5. Bogorad L. 1965; Porphyrins and bile pigments. In Plant Biochemistry pp. 717–760 Bonner J., Varner J. E. Edited by New York: Academic Press;
    [Google Scholar]
  6. Cutting J. A., Schulman H. M. 1969; The site of heme synthesis in soybean root nodules. Biochimica et biophysica acta 192:486–493
    [Google Scholar]
  7. Dilworth M. J., Williams D. C. 1967; Nucleic acid changes in bacteroids of Rhizobium lupini during nodule development. Journal of General Microbiology 48:31–36
    [Google Scholar]
  8. Doss M., Philipp-Dormston W. K. 1971; Porphyrin and heme biosynthesis from endogenous and exogenous ε-aminolaevulinic acid in Escherichia coli, Pseudomonas aeruginosa and Achromobacter metalcaligenes . Hoppe-Seyler’s Zeitschrift für Physiologische Chemie 352:725–733
    [Google Scholar]
  9. Falk J. E., Appleby C. A., Porra R. J. 1959; The nature, function and biosynthesis of the haem compounds and porphyrins of legume root nodules. Symposium of the Society for Experimental Biology 13:73–86
    [Google Scholar]
  10. Hendry G. S., Jordan D. C. 1969; Coproporphyrin excretion by Rhizobium meliloti . Canadian Journal of Microbiology 15:242–244
    [Google Scholar]
  11. Jackson E. K., Evans H. J. 1966; Propionate in heme biosynthesis in soybean nodules. Plant Physiology 41:1673–1680
    [Google Scholar]
  12. Lascelles J. 1964 Tetrapyrrole Biosynthesis and Its Regulation p. 90 New York: W. A. Benjamin;
    [Google Scholar]
  13. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  14. Miyauchi C. 1968; Erythrocyte hemins. I. Separation of erythrocyte hemins by thin-layer chromatography. Proceedings of the Japanese Academy 44:743–747
    [Google Scholar]
  15. Moore D. J., Labbe R. F. 1964; A quantitative assay for urinary porphobilinogen. Clinical Chemistry 10:1105–1111
    [Google Scholar]
  16. Nandi D. L., Shemin D. 1968; δ-Aminolaevulinic acid dehydratase of Rhodopseudomonas spheroides . Journal of Biological Chemistry 243:1236–1242
    [Google Scholar]
  17. Ott D. G., Richmond C. R., Trujillo T. T., Foreman H. 1959; Cab-O-Sil suspensions for liquid scintillation counting. Nucleonics 17:106–108
    [Google Scholar]
  18. Parker C. A., Grove P. L. 1970; The rapid serological identification of rhizobia in small nodules. Journal of Applied Bacteriology 33:248–252
    [Google Scholar]
  19. Parker C. A., Oakley A. E. 1963; Nodule bacteria for two species of Serradella-Ornithopus sativus and Ornithopus compressus . Australian Journal of Experimental Agriculture and Animal Husbandry 3:9–10
    [Google Scholar]
  20. Patterson M. S., Greene R. C. 1965; Measurement of low energy beta-emitters in aqueous solution by liquid scintillation counting of emulsions. Analytical Chemistry 37:854–857
    [Google Scholar]
  21. Smith J. D. 1949; The concentration and distribution of haemoglobin in the root nodules of leguminous plants. Biochemical Journal 44:484–591
    [Google Scholar]
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