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SUMMARY: Four arginine auxotrophs have been obtained in Escherichia coli k12 following treatment with the acridine half-mustard ICR 191. Their properties show that they carry mutations in the E gene of the argECBH cluster, and their reversion patterns indicate that these mutations are frameshifts. The mutations exert no polar effect on the expression of the argC, B and H genes. These results accord with earlier suggestions that E is not transcribed on to the same messenger RNA molecule as C, B and H.
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