1887

Abstract

SUMMARY: Four arginine auxotrophs have been obtained in 12 following treatment with the acridine half-mustard ICR 191. Their properties show that they carry mutations in the gene of the cluster, and their reversion patterns indicate that these mutations are frameshifts. The mutations exert no polar effect on the expression of the and genes. These results accord with earlier suggestions that is not transcribed on to the same messenger RNA molecule as and

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1971-12-01
2022-01-17
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References

  1. Adelberg E. A., Mandel M., Chen G. C. C. 1965; Optimal conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine in Escherichia coli k12. Biochemical and Biophysical Research Communications 18:788–795
    [Google Scholar]
  2. Ashcroft E. 1970 Studies on the arginine cluster in Escherichia coli k-12 Ph.D. Thesis University of Leeds:
    [Google Scholar]
  3. Baich A., Vogel H. J. 1962; N-Acetyl-γ-glutamokinase and N-acetylglutamicγ-semialdehyde dehydrogenase : repressible enzymes of arginine synthesis in Escherichia coli. Biochemical and Biophysical Research Communications 7:491–496
    [Google Scholar]
  4. Baumberg S. 1970; Acetylhistidine as substrate for acetylornithinase: a new system for the selection of arginine regulation mutants in Escherichia coli. Molecular and General Genetics 106:162–173
    [Google Scholar]
  5. Berger H., Brammar W. J., Yanofsky C. 1968; Spontaneous and ICR 191-A-induced frameshift mutations in the A gene of Escherichia coli tryptophan synthetase. Journal of Bacteriology 96:1672–1679
    [Google Scholar]
  6. Cunin R., Elseviers D., Sand G., Freundlich G., Glansdorff N. 1969; On the functional organization of the argECBH cluster of genes in Escherichia coli k-12. Molecular and General Genetics 106:32–47
    [Google Scholar]
  7. Davis B. D. 1948; Isolation of biochemically deficient mutants of bacteria by penicillin. Journal of the American Chemical Society 70:4267
    [Google Scholar]
  8. Elseviers D., Cunin R., Glansdorff N. 1969; Reactivation of arginine genes under the influence of polar mutations. FEBS Letters 3:18–20
    [Google Scholar]
  9. Fink G. R., Martin R. G. 1967; Translation and polarity in the histidine operon. II. Polarity in the histidine operon. Journal of Molecular Biology 30:97–107
    [Google Scholar]
  10. Glansdorff N. 1965; Topography of cotransducible arginine mutations in Escherichia coli k-12. Genetics 51:167–179
    [Google Scholar]
  11. Glansdorff N., Sand G., Verhoef C. 1967; The dual genetic control of ornithine transcarbamylase synthesis in Escherichia coli K-12. Mutation Research 4:743–751
    [Google Scholar]
  12. Jordan E., Saedler H., Starlinger P. 1968; o° and strong-polar mutations in the gal operon are insertions. Molecular and General Genetics 102:353–363
    [Google Scholar]
  13. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  14. Maas W. K. 1961; Studies on repression of arginine biosynthesis in Escherichia coli. Cold Spring Harbor Symposia on Quantitative Biology 26:183–191
    [Google Scholar]
  15. Martin R. G. 1967; Frameshift mutants in the histidine operon of Salmonella typhimurium. Journal of Molecular Biology 26:311–328
    [Google Scholar]
  16. Martin R. G., Whitfield H. J.Jun Berkowitz D. B., Voll M. J. 1966; A molecular model of the phenomenon of polarity. Cold Spring Harbor Symposia on Quantitative Biology 31:215–220
    [Google Scholar]
  17. Newton A. 1970; Isolation and characterization of frameshift mutations in the lac operon. Journal of Molecular Biology 49:589–601
    [Google Scholar]
  18. Oeschger N. S., Hartman P. E. 1970; ICR-induced frameshift mutations in the histidine operon of Salmonella. Journal of Bacteriology 101:490–504
    [Google Scholar]
  19. Ratner S. 1957; Preparation and determination of argininosuccinic acid. In Methods in Enzymology III pp. 643–647 Colowick S. P., Kaplan N. O. Edited by New York: Academic Press;
    [Google Scholar]
  20. Riyasaty S., Atkins J. F. 1968; External suppression of a frameshift mutant in Salmonella. Journal of Molecular Biology 34:541–557
    [Google Scholar]
  21. Shapiro J. A. 1969; Mutations caused by the insertion of genetic material into the galactose operon of Escherichia coli. Journal of Molecular Biology 40:93–105
    [Google Scholar]
  22. Siegel E. C., Bryson V. 1967; Mutatorgene of Escherichia coli b. Journal of Bacteriology 94:38–47
    [Google Scholar]
  23. Taylor A. L. 1970; Current linkage map of Escherichia coli. Bacteriological Reviews 34:155–175
    [Google Scholar]
  24. Theil E. C., Forsyth G. W., Jones E. E. 1969; Expression of the arginine regulon of Escherichia coli w: evidence for a second regulatory gene. Journal of Bacteriology 99:269–273
    [Google Scholar]
  25. Vogel H. J., Baumberg S., Bacon D. F., Jones E. E., Unger L., Vogel R. H. 1967; Gene-ribosome-enzyme organization in the arginine system of Escherichia coli. In Organizational Biosynthesis pp. 223–234 Vogel H. J., Lampen J. O., Bryson V. Edited by New York: Academic Press;
    [Google Scholar]
  26. Vogel H. J., Bonner D. M. 1956; Acetylomithinase of Escherichia coli: Partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  27. Vogel H. J., McLellan W. L. 1970; N-Acetylglutamicγ-semialdehyde dehydrogenase (Escherichia coli). In Methods in Enzymology XVIIA pp. 255–260 Tabor H., Tabor C. W. Edited by New York: Academic Press;
    [Google Scholar]
  28. Whitfield H. J.Jun Martin R. G., Ames B. N. 1966; Classification of aminotransferase (C gene) mutants in the histidine operon. Journal of Molecular Biology 21:335–355
    [Google Scholar]
  29. Yourno J., Heath S. 1969; Nature of the hisD3018 frameshift mutation in Salmonella typhimurium. Journal of Bacteriology 100:460–468
    [Google Scholar]
  30. Zipser D., Zabell S., Rothman J., Grodzicker T., Wenk M. 1970; Fine structure of the gradient of polarity in the z gene of the lac operon of Escherichia coli. Journal of Molecular Biology 49:251–254
    [Google Scholar]
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