1887

Abstract

SUMMARY: produced hydrogen, CO and acetyl phosphate from pyruvate by a clostridial-type thioclastic reaction which required coenzyme A and ferredoxin for activity. Hydrogen production from formate did not require ferredoxin since crude formate hydrogenlyase preparations functioned when depleted of ferredoxin.

With fractionated components of the enzymic system, ferredoxin or viologen dyes restored activity. No evidence for the presence of a coliform-type thioclastic reaction (pyruvate-formate lyase) was found. Reduction of NADP by hydrogen or formate was ferredoxin-dependent. Acetaldehyde and CO were produced from pyruvate by a yeast-type decarboxylase which required thiamine pyrophosphate and Mg ions for activity. Extracellular pH influenced the pathways of pyruvate metabolism which were reflected by the molar growth yields for glucose; the thioclastic reaction was predominant at alkaline pH.

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1971-12-01
2022-05-20
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References

  1. Arbuthnott J. P., Bauchop T., Dawes E. A. 1960; A clastic fission of pyruvic acid in Zymosarcina ventriculi . Biochemical Journal 76:12P
    [Google Scholar]
  2. Barker S. A., Somers P. J. 1966; A spectrophotometric method for the determination of formic acid in the periodate oxidation of carbohydrates. Carbohydrate Research 3:220–224
    [Google Scholar]
  3. Bauchop T., Dawes E. A. 1959; Metabolism of pyruvic and formic acids by Zymosarcina ventriculi . Biochimica et biophysica acta 36:294–296
    [Google Scholar]
  4. Bauchop T., Dawes E. A. 1968; The metabolism of formic acid by Sarcina ventriculi . Journal of General Microbiology 52:195–203
    [Google Scholar]
  5. Bauchop T., Elsden S. R. 1960; The growth of micro-organisms in relation to their energy supply. Journal of General Microbiology 23:457–469
    [Google Scholar]
  6. Canale-Parola E. 1961 Ph.D. Dissertation University of Illinois, Urbana:
  7. Canale-Parola E. 1970; Biology of the sugar-fermenting sarcinae. Bacteriological Reviews 34:82
    [Google Scholar]
  8. Canale-Parola E., Wolfe R. S. 1960; Studies on Sarcina ventriculi, II. Nutrition. Journal of Bacteriology 79:860–862
    [Google Scholar]
  9. Chase J. F. A., Middleton B., Tubbs P. K. 1966; A coenzyme A analogue, desulpho-CoA; preparation and effects on various enzymes. Biochemical and Biophysical Research Communications 23:208–213
    [Google Scholar]
  10. French C. S., Milner H. W. 1955; Disintegration of bacteria and small particles by high-pressure extrusion. In Methods in Enzymology 1 pp. 64–67 Colowick S. P., Kaplan N. O. Edited by New York: Academic Press;
    [Google Scholar]
  11. Friedemann T. E., Haugen G. E. 1943; Pyruvic acid. II. The determination of keto acids in blood and urine. Journal of Biological Chemistry 147:415–422
    [Google Scholar]
  12. Gornall A. G., Bardawill C. J., David M. M. 1949; Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  13. Hespell R. B., Joseph R., Mortlock R. P. 1969; Requirement for coenzyme A in the phosphoroclastic reaction of anaerobic bacteria. Journal of Bacteriology 100:1328–1334
    [Google Scholar]
  14. Higgins T. E., Johnson M. J. 1970; Pathways of anaerobic acetate utilization in Escherichia coli and Aerobacter cloacae . Journal of Bacteriology 101:885–891
    [Google Scholar]
  15. Hohorst H. J. 1963; l (+) Lactate. Determination with lactic dehydrogenase and DPN. In Methods of Enzymatic Analysis pp. 266–270 Bergmeyer H. U. Edited by New York and London: Academic Press;
    [Google Scholar]
  16. Jungermann K., Kirchniawy H., Thauer R. K. 1970; Ferredoxin dependent CO2 reduction to formate in Clostridium Pasteurianum . Biochemical and Biophysical Research Communications 41:682–689
    [Google Scholar]
  17. King T. E., Cheldelin V. H. 1954; Pyruvic carboxylase of Acetobacter suboxydans . Journal of Biological Chemistry 208:821–831
    [Google Scholar]
  18. Kluyver A. J. 1931 The Chemical Activities of Micro-organisms p. 39 London: University of London Press;
    [Google Scholar]
  19. Lindmark D. G., Paolella P., Wood N. P. 1969; The pyruvate-formatelyase system of Streptococcus faecalis . Journal of Biological Chemistry 244:3605–3612
    [Google Scholar]
  20. Lipmann F., Tuttle L. C. 1945; A specific micromethod for the determination of acyl phosphates. Journal of Biological Chemistry 159:21–28
    [Google Scholar]
  21. Mayhew S. G. 1966 Studies on ferredoxin and rubredoxin Ph.D. Thesis University of Sheffield:
    [Google Scholar]
  22. Milhaud G., Aubert J. P., van Niel C. B. 1956; Étude de la glycolyse de Zymosarcina ventriculi . Annales de L’Institut Pasteur 91:363–368
    [Google Scholar]
  23. Morey A. V., Juni E. 1968; Studies on the nature of the binding of thiamine pyrophosphate to enzymes. Journal of Biological Chemistry 243:3009–3019
    [Google Scholar]
  24. Mortenson L. E., Valentine R. C., Carnahan J. E. 1962; An electron transport factor from Clostridium Pasteurianum . Biochemical and Biophysical Research Communications 7:448–452
    [Google Scholar]
  25. Neish A. C. 1952 Analytical methods for bacterial fermentations National Research Council of Canada Report no:46-8-3
    [Google Scholar]
  26. Payne W. J. 1970; Energy yields and growth of heterotrophs. Annual Review of Microbiology 24: pp. 17–52
    [Google Scholar]
  27. Smit J. 1930 Die Gärungssarcine. MonographiePflanzenforschung Heft14 Jena: Gustav Fischer;
    [Google Scholar]
  28. Stephenson M. P., Dawes E. A. 1970; The role of ferredoxin in formate metabolism of Sarcina ventriculi . Journal of General Microbiology 60:vi–vii
    [Google Scholar]
  29. Valentine R. C., Brill W. J., Wolfe R. S. 1962; Role of ferredoxin in pyridine nucleotide reduction. Proceedings of the National Academy of Sciences of the United States of America 48:1856–1860
    [Google Scholar]
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