1887

Abstract

SUMMARY: produced hydrogen, CO and acetyl phosphate from pyruvate by a clostridial-type thioclastic reaction which required coenzyme A and ferredoxin for activity. Hydrogen production from formate did not require ferredoxin since crude formate hydrogenlyase preparations functioned when depleted of ferredoxin.

With fractionated components of the enzymic system, ferredoxin or viologen dyes restored activity. No evidence for the presence of a coliform-type thioclastic reaction (pyruvate-formate lyase) was found. Reduction of NADP by hydrogen or formate was ferredoxin-dependent. Acetaldehyde and CO were produced from pyruvate by a yeast-type decarboxylase which required thiamine pyrophosphate and Mg ions for activity. Extracellular pH influenced the pathways of pyruvate metabolism which were reflected by the molar growth yields for glucose; the thioclastic reaction was predominant at alkaline pH.

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1971-12-01
2024-12-07
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