Growth of Unicellular Forms of the Fungus and Analysis of the Chemical Composition of their Walls Free

Abstract

SUMMARY: When is grown in shaking culture in certain media, two large unicellular forms are produced. The walls isolated from these are similar both qualitatively and quantitatively except for their mannose content, one form containing almost twice the amount as the other (17·5 versus 9·6%). Glucose, the predominant monosaccharide obtained by acid hydrolysis, accounts for more than half (52 to 60%) of the weight of the walls. The remainder is composed of galactose (6%), hexosamine (6%), lipid (8 to 10%), and protein (8 to 10%).

Walls of the unicellular forms contain more glucose and protein than those of the filamentous form, but less hexosamine and lipid. Glusulase releases all of the glucose from walls of the filaments, but only half of the glucose from walls of the unicells.

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/content/journal/micro/10.1099/00221287-69-2-253
1971-12-01
2024-03-29
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References

  1. Bartnicki-Garcia S. 1968; Cell wall chemistry, morphogenesis, and taxonomy of fungi. Annual Review of Microbiology 22:87–108
    [Google Scholar]
  2. Guarino A. 1967; Cordycepin. In Antibiotics 1 pp. 468–480 Gottlieb D., Shaw P. Edited by New York: Springer-Verlag;
    [Google Scholar]
  3. Kleinsmith K., Allfrey V. 1969; Nuclear phosphoproteins. I. Isolation and characterization of a phosphoprotein fraction from calf thymus nuclei. Biochimica et biophysica acta 175:123–135
    [Google Scholar]
  4. Kredich N., Guarino A. 1960; An improved method of isolation and identification of cordycepin. Biochimica et biophysica acta 41:363–365
    [Google Scholar]
  5. Marks D., Keller B., Guarino A. 1969; The composition of the cell wall fraction of the fungus, Cordyceps militaris. Biochimica et biophysica acta 183:58–64
    [Google Scholar]
  6. Thom C., Raper K. 1945 In A Manual of the Aspergilli p. 32 Baltimore: Williams and Wilkins;
    [Google Scholar]
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