%0 Journal Article %A Lacey, R. W. %T Transfer of Tetracycline-resistance between Strains of Staphylococcus aureus in Mixed Cultures %D 1971 %J Microbiology, %V 69 %N 2 %P 229-237 %@ 1465-2080 %R https://doi.org/10.1099/00221287-69-2-229 %I Microbiology Society, %X SUMMARY: A plasmid conferring tetracycline-resistance from a clinical isolate of Staphylococcus aureus (5401) was transferred to a wide variety of other strains by mixed culture in nutrient broth. Strain 609 converted to tet-r by this method could transfer tet-r efficiently (up to 10−3 in 12 h.) to other strains. Co-factor studies on this donor suggested that transfer of tet-r is mediated by phage transduction. However, filtrates of the donor culture contained few particles able to transfer tetracycline-resistance. It is probable that the transfer occurs by short-lived or cell-bound phage particles. Filtrates of the donor strain obtained after induction with mitomycin C were able to transduce tetracycline-resistance, but at low frequency. The gene(s) for tetracycline-resistance in these strains is probably borne by a plasmid, since they segregate tetracycline-sensitive derivatives. Ultraviolet (u.v.) light-inactivation studies on transduction of tet-r support this conclusion. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-69-2-229