SUMMARY: A plasmid conferring tetracycline-resistance from a clinical isolate of (5401) was transferred to a wide variety of other strains by mixed culture in nutrient broth. Strain 609 converted to by this method could transfer efficiently (up to 10 in 12 h.) to other strains. Co-factor studies on this donor suggested that transfer of is mediated by phage transduction. However, filtrates of the donor culture contained few particles able to transfer tetracycline-resistance. It is probable that the transfer occurs by short-lived or cell-bound phage particles. Filtrates of the donor strain obtained after induction with mitomycin C were able to transduce tetracycline-resistance, but at low frequency.

The gene(s) for tetracycline-resistance in these strains is probably borne by a plasmid, since they segregate tetracycline-sensitive derivatives. Ultraviolet (u.v.) light-inactivation studies on transduction of support this conclusion.


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