1887

Abstract

SUMMARY: A plasmid conferring tetracycline-resistance from a clinical isolate of (5401) was transferred to a wide variety of other strains by mixed culture in nutrient broth. Strain 609 converted to by this method could transfer efficiently (up to 10 in 12 h.) to other strains. Co-factor studies on this donor suggested that transfer of is mediated by phage transduction. However, filtrates of the donor culture contained few particles able to transfer tetracycline-resistance. It is probable that the transfer occurs by short-lived or cell-bound phage particles. Filtrates of the donor strain obtained after induction with mitomycin C were able to transduce tetracycline-resistance, but at low frequency.

The gene(s) for tetracycline-resistance in these strains is probably borne by a plasmid, since they segregate tetracycline-sensitive derivatives. Ultraviolet (u.v.) light-inactivation studies on transduction of support this conclusion.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-69-2-229
1971-12-01
2021-08-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/69/2/mic-69-2-229.html?itemId=/content/journal/micro/10.1099/00221287-69-2-229&mimeType=html&fmt=ahah

References

  1. Altenbern R. A. 1967; Genetic studies of pigmentation of Staphylococcus aureus . Canadian Journal of Microbiology 13:389–395
    [Google Scholar]
  2. Arber W. 1960; Transduction of chromosomal genes and episomes in Escherichia coli . Virology 11:273–288
    [Google Scholar]
  3. Asheshov E. H. 1966; Chromosomal location of the genetic elements controlling penicillinase production in a strain of Staphylococcus aureus . Nature; London: 210804–806
    [Google Scholar]
  4. Asheshov E. H. 1969; The genetics of penicillinase production in Staphylococcus aureus strain PS80. Journal of General Microbiology 59:289–301
    [Google Scholar]
  5. Birnsungl M. A., Shooter R. A., Hunt M. F. 1952; Sensitivity to five antibiotics of strains of Staph. pyogenes isolated from out-patients. British Medical Journal 2:253–254
    [Google Scholar]
  6. Blair J. E., Williams R. E. O. 1961; Phage typing of staphylococci. Bulletin of the World Health Organisation 24:771–784
    [Google Scholar]
  7. Bülow P. 1970; A new epidemic phage type of Staphylococcus aureus. 5. Epidemic spread of phages among Danish hospital staphylococci. Acta pathologica et microbiologica scandinavica 78B:29–40
    [Google Scholar]
  8. Dyke K. G. H., Parker M. T., Richmond H. M. 1970; Penicillinase production and metal-ion resistance in Staphylococcus aureus cultures isolated from hospital patients. Journal of Medical Microbiology 3:125–136
    [Google Scholar]
  9. Elek S. D. 1959 Staphylococcus pyogenes and its relation to Disease p. 472 Edinburgh: Livingstone;
    [Google Scholar]
  10. Gonzalez C., Sierra G. 1961; Lipolyte activity of some anaerobic bacteria. Nature; London: 189601–602
    [Google Scholar]
  11. Jarolmen H., Bondi A., Crowell R. L. 1965; Transduction of Staphylococcus aureus to tetracycline resistance in vivo . Journal of Bacteriology 89:1286–1290
    [Google Scholar]
  12. Jevons M. P., John M., Parker M. T. 1966; Cultural characters of a newly recognized group of hospital staphylococci. Journal of Clinical Pathology 19:305–312
    [Google Scholar]
  13. Lacey R. W. 1971; High-frequency transfer of neomycin resistance between naturally-occurring strains of Staphylococcus aureus . Journal of Medical Microbiology 4:73–84
    [Google Scholar]
  14. Lacey R. W., Alder V. G., Gillespie W. A. 1970; The survival of Staphylococcus aureus on human skin. An investigation using mixed cultures. British Journal of Experimental Pathology 51:305–313
    [Google Scholar]
  15. McDonald S. 1966; Transduction of antibiotic resistance in Staphylococcus aureus . Lancet 2:1107–1110
    [Google Scholar]
  16. Mitsuhashi S., Oshima H., Kawaharada U., Hashimoto H. 1965; Drug resistance of staphylococci: 1. Transduction of tetracycline resistance with phage-lysates obtained from multiply resistant staphylococci. Journal of Bacteriology 89:967–976
    [Google Scholar]
  17. Novick R. P. 1967; Properties of a cryptic high-frequency transducing phage in Staphyloccocus aureus . Virology 33:155–166
    [Google Scholar]
  18. Novick R. P., Morse S. I. 1967; In vivo transmission of drug resistance factors between strains of Staphylococcus aureus . Journal of Experimental Medicine 125:45–59
    [Google Scholar]
  19. Pattee P. A., Baldwin J. N. 1961; Transduction of resistance to chlortetracycline and novobiocin in Staphylococcus aureus . Journal of Bacteriology 82:875–881
    [Google Scholar]
  20. Poston S. M. 1966; Cellular location of the genes controlling penicillinase production and resistance to streptomycin and tetracycline in a strain of Staphylococcus aureus . Nature; London: 210802–804
    [Google Scholar]
  21. Report of the sub-committee on phage-typing of staphylococci of the international committee on nomenclature of bacteria, Moscow, July 1966 1967 International Journal of Systemic Bacteriology 17113–125
    [Google Scholar]
  22. Richmond M. H. 1969; Extrachromosomal elements and the spread of antibiotic resistance in bacteria. Biochemical Journal 113:225–234
    [Google Scholar]
  23. Rountree P. M. 1949; The phenomenon of lysogenicity in staphylococci. Journal of General Microbiology 3:153–163
    [Google Scholar]
  24. Rountree P. M. 1951; The role of certain electrolytes in the adsorption of staphylococcal bacteriophages. Journal of General Microbiology 5:673–680
    [Google Scholar]
  25. Williams R. E. O., Rippon J. E. 1952; Bacteriophage typing of Staphylococcus aureus . Journal of Hygiene 50:320–353
    [Google Scholar]
  26. Willis A. T., Turner G. C. 1963; Staphyloccoci in the hospital environment. Journal of Pathology and Bacteriology 85:395–405
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-69-2-229
Loading
/content/journal/micro/10.1099/00221287-69-2-229
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error