The Effects of Technical Chlordane on Growth and Energy Metabolism of Streptococcus faecalis and Mycobacterium phlei: a Comparison with Bacillus subtilis
SUMMARY: The growth of Mycobacterium phlei and Bacillus subtilis either on plate culture or in liquid medium is completely inhibited by technical chlordane at identical concentrations. NADH oxidation by membrane fragments and oxidative phosphorylation by crude extracts of M. phlei are only partially inhibited by incubation with chlordane at concentrations equal to or greater than the minimum levels required to completely inhibit growth.
Streptococcus faecalis is relatively insensitive to technical chlordane, bacteriosta-sis only being achieved at chlordane concentrations some five-to tenfold greater than those required with Bacillus subtilis and Mycobacterium phlei. The energy metabolism of S. faecalis is relatively simple since it contains neither a cytochrome-mediated electron transport chain nor a functional TCA cycle. Fermentation of d-glucose by soluble protein extracts, Na-K-ATPase and cation permeability of the plasma membrane are not sensitive to chlordane at concentrations that stop growth in liquid culture. Inhibition of fermentation by growing cultures, upon addition of chlordane, may be a reflection of the inhibition of biosynthetic reactions that recycle ATP.
AbramsA.1960; Metabolically dependent penetration of oligosaccharides into bacterial cells and protoplasts. Journal of Biological Chemistry 235:1281–1285
ColvinH. J., PhillipsA. T.1968; Inhibition of electron transport and cholinesterases by endrin. Bulletin of Environmental Contamination and Toxicology 3:106–115
FaustP. J., VandemarkP. J.1970; Phosphorylation coupled to NADH oxidation with fumarate in Streptococcus faecalis 10 C1. Archives of Biochemistry and Biophysics 137:392–398
FletcherD. W., BollenW. B.1954; The effects of aldrin on soil micro-organisms and some of their activities related to soil fertility. Applied Microbiology 2:349–354
GallinJ. I., VandemarkP. J.1964; Evidence for oxidative phosphorylation in Streptococcus faecalis10 C1. Biochemical and Biophysical Research Communications 17:630–635
GornallA. G., BardawillC. J., DavidM. M.1949; Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry 177:751–766
HaroldF. M., BaardaJ. R., BaronC., AbramsA.1969; Inhibition of membrane bound ATPase and cation transport of Streptococcus faecalis by N,N-dicyclohexylcarbodiimide. Journal of Biological Chemistry 244:2261–2268
HaroldF. M., PavlasovaE., BaardaJ. R.1970; A transmembrane pH gradient in Streptococcus faecalis: origin, and dissipation by proton conductors and N,N-dicyclohexylcarbodiimide. Biochemica et biophysica acta 196:235–244
MathewsM. M., SistromW. R.1959; Intracellular location of carotenoid pigments and some respiratory enzymes in Sarcina lutea. Journal of Bacteriology 78:778–787
MorrisonP. E., BrownA. W. A.1954; The effects of insecticides on cytochrome oxidase obtained from the American cockroach. Journal of Economic Entomology 47:723–730
NelsonB. D., WilliamsC.1971; Action of cyclodiene pesticides on oxidative metabolism in the yeast Saccharomyces cerevisiae. Journal of Food and Agricultural Chemistry 19:339–341
TrudgillP. W., WiddusR., ReesJ. S.1971; Effects of organochlorine insecticides on bacterial growth, respiration and viability. Journal of General Microbiology 69:1–13
WiddusR., TrudgillP. W., MaliszewskiM. J.1971; The effects of technical chlordane on energy metabolism of Bacillus subtilis. Journal of General Microbiology 69:15–22
The Effects of Technical Chlordane on Growth and Energy Metabolism of Streptococcus faecalis and Mycobacterium phlei: a Comparison with Bacillus subtilis