1887

Abstract

SUMMARY: Two sprays of streptomycin (1000 g./ml.) considerably reduce sporulation of the hop downy mildew fungus, on leaves of primary systemically infected shoots. Streptomycin markedly inhibited germination of its sporangia Toxicity was dependent on sporangial concentration, incubation temperature and the cleanliness of the suspension but was independent of sporangial age. There were no marked differences in sensitivity to streptomycin between sporangia from five different isolates of Two commercial formulations of streptomycin were as inhibitory to sporangial germination as pure streptomycin sulphate. Sublethal concentrations of streptomycin interfered with cleavage of sporangial cytoplasm and increased the proportion of multiflagellate zoospores.The proportion of multiflagellate multinucleate zoospores liberated was dependent on streptomycin concentration. Germination of multiflagellate zoospores was observed. Streptomycin was very inhibitory to zoospore germination but had little effect on zoospore motility and germ-tube growth at concentrations which caused a high incidence of cyst lysis. Streptomycin was as toxic to sporangia of as to those of whereas sporangia of and were less sensitive to the antibiotic.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-69-1-117
1971-11-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/69/1/mic-69-1-117.html?itemId=/content/journal/micro/10.1099/00221287-69-1-117&mimeType=html&fmt=ahah

References

  1. Altman J., Davis B. H. 1958; Experiments on the control of downy mildew of broccoli and bacterial spot of lima bean with streptomycin. Plant Disease Reporter 42:416–419
    [Google Scholar]
  2. Anderson P. J. 1956; Streptomycin for control of blue mould and bed rot of tobacco. Phytopathology 46:240
    [Google Scholar]
  3. Ark P. A., Thompson J. B. 1957; Control of downy mildew of cucumber with antibiotics. Plant Disease Reporter 41:452–454
    [Google Scholar]
  4. Ark P. A., Wilson E. M. 1956; Movement of streptomycin into plant tissues from streptomycin-pyrophyllite formulations. Phytopathology 46:634
    [Google Scholar]
  5. Bliss C. I. 1935; The calculation of the dosage-mortality curve. Annals of Applied Biology 22:134–167
    [Google Scholar]
  6. Boyd R. E. 1968 Mutational studies on Phytophthorainfestans (Mont.) de Bary Ph.D. Thesis University of Hull:
    [Google Scholar]
  7. Clarke D. D. 1966; Factors affecting the development of single zoospore colonies of Phytophthora infestans. Transactions of the British Mycological Society 49:177–184
    [Google Scholar]
  8. Coe D. M. 1955; Streptomycin as a control for downy mildew of cucurbits. Plant Disease Reporter 39:729–730
    [Google Scholar]
  9. Coley-Smith J. R. 1966; Early-season control of hop downy mildew, Pseudoperonospora humuli (Miy. & Tak.) Wilson, with streptomycin and protectant fungicides in severely infected plantings. Annals of Applied Biology 57:183–191
    [Google Scholar]
  10. Coley-Smith J. R., Beard F. H. 1961; Pathology Section. Review of the year’s work. In Wye College Department of Hop Research Annual Report for 1960 pp. 23–25
    [Google Scholar]
  11. Coley-Smith J. R., Royle D. J. 1965; Pathology Section.Ten year review. In Wye College Department of Hop Research Annual Report for 1964 pp. 66–72
    [Google Scholar]
  12. Crosse R., McWilliam R., Rhodes A., Dunn A. T. 1960; Antifungal action of streptomycin-copper chelate against Phytophthora infestans on tomato (Lycopersicon esculentum). Annals of Applied Biology 48:270–278
    [Google Scholar]
  13. Cruickshank I. A. M., Mueller K. O. 1957; Water-relations and sporulation of Peronospora tabacina Adam. Nature; London: 18044–45
    [Google Scholar]
  14. Davies J., Gorini L., Davis B. D. 1965; Misreading of RNA codewords induced by aminoglycoside antibiotics. Molecular Pharmacology 1:93–106
    [Google Scholar]
  15. Dubin D. T., Hancock R., Davis B. D. 1963; The sequence of some effects of streptomycin in Escherichia coli. Biochimica et biophysica acta 74:476–489
    [Google Scholar]
  16. Garrett R. G., Tomlinson J. A. 1967; Isolate differences in Olpidium brassicae. Transactions of the British Mycological Society 50:429–435
    [Google Scholar]
  17. Gottlieb D. 1952; Disappearance of antibiotics from soil. Phytopathology 42:9
    [Google Scholar]
  18. Griffin M. J. 1969 Some effects of streptomycin on sporangia of Pseudoperonospora humuli (Miy. &Tak.) Wilson Ph.D. Thesis University of Hull:
    [Google Scholar]
  19. Griffin M. J., Coley-Smith J. R. 1968; The establishment of hop tissue cultures and their infection by downy mildew Pseudoperonospora humuli (Miy. & Tak.) Wilson under aseptic conditions. Journal of General Microbiology 53:231–236
    [Google Scholar]
  20. Grosso J. J. 1954; Control of tobacco blue mold by antibiotics. Plant Disease Reporter 38:333
    [Google Scholar]
  21. Ho H. H., Hickman C. J. 1967; Asexual reproduction and behaviour of zoospores of Phytophthora megasperma var. sojae. Canadian Journal of Botany 45:1963–1981
    [Google Scholar]
  22. Horner C. E. 1963; Chemotherapeutic effects of streptomycin on establishment and progression of systemic downy mildew infection in hops. Phytopathology 53:472–474
    [Google Scholar]
  23. Horner C. E., Maier C. R. 1957; Antibiotics eliminate systemic downy mildew from hops. Phytopathology 47:525
    [Google Scholar]
  24. King J. E., Colhoun J., Butler R. D. 1968; Changes in the ultrastructure of sporangia of Phytophthora infestans associated with indirect germination and ageing. Transactions of the British Mycological Society 51:269–281
    [Google Scholar]
  25. Kole A. P. 1955; Some observations on the zoospores from the zoosporangia of Plasmodiophora brassicae Woron. Tijdschrift over Plantenziekten 61:159–162
    [Google Scholar]
  26. McLean R. C., Cook W. R. I. 1952 Plant Science Formulae London: Macmillan;
    [Google Scholar]
  27. Maier C. R. 1959; Effects of streptomycin sulphate on the hop downy mildew fungus in vivo. Phytopathology 49:545
    [Google Scholar]
  28. Maier C. R. 1960; Streptomycin absorption, translocation, and retention in hops. Phytopathology 50:351–356
    [Google Scholar]
  29. Müller K. O., Mackay J. H., Friend J. N. 1954; Effect of streptomycin on the host-pathogen relationship of a fungal pathogen. Nature; London: 174878–879
    [Google Scholar]
  30. Ogawa J. M., McCain A. H., Hall D. M. 1960; Streptomycin absorption in diseased and healthy hop tissues and its effect on the hop downy mildew organism and mildew development. Phytopathology 50:278–280
    [Google Scholar]
  31. Pelletier E. N., Hilborn M. T. 1953; Blossom and twig-blight of low-bush blueberry. Phytopathology 43:481
    [Google Scholar]
  32. Royle D. J. 1970; Infection periods in relation to the natural development of hop downy mildew (Pseudoperonospora humuli). Annals of Applied Biology 66:281–291
    [Google Scholar]
  33. Schicke P. 1961; Beobachtungen zur systemischen Fungizidwirkung von Streptomycin. Mededelingen van de Landbouwhoogeschool en de Opzoekingsstations van de Staat te Gent 26:1370–1377
    [Google Scholar]
  34. Shaw D. S., Elliot C. G. 1968; Streptomycin resistance and morphological variation in Phytophthora cactorum. Journal of General Microbiology 51:75–84
    [Google Scholar]
  35. Siminoff P., Gottlieb D. 1951; The production and role of antibiotics in the soil. I. The fate of streptomycin. Phytopathology 41:420–430
    [Google Scholar]
  36. Venis M. A. 1969; Similarities in action between streptomycin and hordatine, an antifungal factor in barley. Phytochemistry 8:1193–1197
    [Google Scholar]
  37. Vörös J. 1963; Mode of action of selective fungistatic effect of streptomycin. Nature; London: 1991110–1111
    [Google Scholar]
  38. Vörös J. 1965; Streptomycin sensitivity of Oomycetes due to the increased absorption of streptomycin by their mycelia. Phytopathologische Zeitschrift 54:249–257
    [Google Scholar]
  39. Vörös J., Kiraly Z., Farkas G. L. 1957; Role of polyphenolase in streptomycin-induced resistance to Phytophthora in potato. Science; New York: 1261178
    [Google Scholar]
  40. Vujičić R., Colhoun J. 1966; Asexual reproduction in Phytophthora erythroseptica. Transactions of the British Mycological Society 49:245–254
    [Google Scholar]
  41. Wilcoxon F., McCallan S. E. A. 1939; Theoretical principles underlying laboratory toxicity tests of fungicides. Contributions. Boyce Thompson Institute for Plant Research 10:329–338
    [Google Scholar]
  42. Zaumeyer W. J., Doolittle S. P. 1956; Control of late blight on tomato with streptomycin. Phytopathology 46:32
    [Google Scholar]
  43. Zaumeyer W. J., Wester R. E. 1956; Control of downy mildew of lima beans with streptomycin. Phytopathology 46:32
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-69-1-117
Loading
/content/journal/micro/10.1099/00221287-69-1-117
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error