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Growth of Mycobacterium lepraemurium in Diffusion Chambers Containing Human Embryonic Skin Cells and in Cell-free Chambers
Abstract
Summary: Mycobacterium lepraemurium grew in diffusion chambers containing human embryonic skin cells maintained in mice, guinea pigs and Petri plate monolayer cultures of human embryonic skin cells. Growth also occurred in diffusion chambers without skin cells maintained in mice and guinea pigs. A 28·8-fold increase in the number of bacilli was obtained when chambers containing skin cells were incubated in mice for 50 days and a 14·2-fold increase when incubated in guinea pigs. Significantly, chambers without skin cells gave a 12·2-fold increase in the number of acid-fast bacilli when maintained in mice for 50 days but only a 5·9-fold increase when maintained in guinea pigs for 50 days.
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© Society for General Microbiology 1971