1887

Abstract

SUMMARY

Polymyxin B and the non-ionic surfactant polysorbate 80 acted synergistically against with respect to leakage, death and lysis. Polysorbate 80 alone was non-toxic. The enhancement of polymyxin occurred throughout the polysorbate concentration range (0·0001 to 0#x00B7;250%, v/v). No sudden change in the pattern of enhancement occurred near the critical micelle concentration of polysorbate. The onset of action of polysorbate was prompt, implying a direct physical effect on the bacteria. Exposure of bacteria to this surfactant did not cause an increase in uptake of polymyxin. It is proposed that polysorbate 80 alters the outer lipid structure of the envelope of allowing easier access of polymyxin to the underlying membrane.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-68-3-367
1971-11-01
2022-05-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/68/3/mic-68-3-367.html?itemId=/content/journal/micro/10.1099/00221287-68-3-367&mimeType=html&fmt=ahah

References

  1. Bernheim F. 1963; Factors which affect the size of organisms and the optical density of suspensions of Pseudomonas aeruginosa and Escherichia coli. Journal of General Microbiology 30:53–58
    [Google Scholar]
  2. Buss E. A., Warth P. T. 1950; The effect of surface-active agents on antibiotics: an informal report. Proceedings of the New York Academy of Science 53:38
    [Google Scholar]
  3. Bloch H., Noll H. 1953; Studies on the virulence of tubercle bacilli. Variations in virulence affected by Tween 80 and thiosemicarbazone. Journal of Experimental Medicine 97:1–16
    [Google Scholar]
  4. Brown M. R. W., Farwell J. A., Rosenbluth S. A. 1969; Use of membrane filters for measurement of 260 mµ absorbing substances from bacterial cells. Analytical Biochemistry 27:484–491
    [Google Scholar]
  5. Brown M. R. W., Melling J. 1969; Role of divalent cations in the action of polymyxin B and EDTA on Pseudomonas aeruginosa. Journal of General Microbiology 59:263–274
    [Google Scholar]
  6. Brown M. R. W., Richards R. M. E. 1964; The effect of Tween 80 on the resistance of Pseudomonas aeruginosa to chemical inactivation. Journal of Pharmacy and Pharmacology 16:51–55
    [Google Scholar]
  7. Brown M. R. W., Watkins W. 1970; Low magnesium and phospholipid content of cell walls of Pseudomonas aeruginosa resistant to polymyxin. Nature; London: 3271360–1361
    [Google Scholar]
  8. Brown M. R. W., Winsley B. E. 1969; Effect of polysorbate 80 on cell leakage and viability of Pseudomonas aeruginosa exposed to rapid changes of pH, temperature and tonicity. Journal of General Microbiology 56:99–107
    [Google Scholar]
  9. Dastugue G., Bastide P., Decros M. 1959; Influence of some surface-active agents on membrane permeability. In studies with larvae of amphibia. Année pharmaceutique 17:269–278
    [Google Scholar]
  10. Fisher M. W. 1948; Sensitivity of tubercle bacilli to streptomycin. American Review of Tuberculosis and Pulmonary Diseases 57:58–62
    [Google Scholar]
  11. Fletcher F., Epstein C., Jewell P. I. 1953; The antimycobacterial activity of tissue extracts and surface-active agents in Dubos’s medium. Journal of General Microbiology 8:323–329
    [Google Scholar]
  12. Garrett E. R., Brown M. R. W. 1963; The action of tetracycline and chloramphenicol alone and in admixture on the growth of Escherichia coli. Journal of Pharmacy and Pharmacology 15:185–191
    [Google Scholar]
  13. Godson G. N., Sinsheimer R. L. 1967; Lysis of Escherichia coli with a neutral detergent. Biochemica et biophysica acta 149:476–488
    [Google Scholar]
  14. Goodhart F. W., Martin A. N. 1962; Solubilization of benzoic acid derivatives by polyoxyethylene stearates. Journal of Pharmaceutical Sciences 51:50–54
    [Google Scholar]
  15. Guttman D., Higuchi T. 1956; Possible complex formation between macromolecules and certain pharmaceuticals X. Journal of the American Pharmaceutical Association Scientific Edition 40:390–393
    [Google Scholar]
  16. Kirby W. W. M., Dubos R. J. 1947; Effect of penicillin on the tubercle bacillus in vitro. Proceedings of the Society for Experimental Biology and Medicine 66:120
    [Google Scholar]
  17. Lacey B. W. 1958; Chemotherapeutic Synergy. 8th Symposium of the Society for General Microbiology Cambridge: Cambridge University Press;
    [Google Scholar]
  18. Lorian V. 1967; Comparison OF mycobacterial cultures on agar and Lowenstein medium. Applied Microbiology 15:1202–1205
    [Google Scholar]
  19. Newton B. A. 1954; Site of action of polymyxin on Psedomonas aeruginosa: antagonism by cations. Journal of General Microbiology 10:491–499
    [Google Scholar]
  20. Newton B. A. 1958; Surface-active bactericides. 8th Symposium of the Society for General Microbiology Cambridge: Cambridge University Press;
    [Google Scholar]
  21. Paunescu E., Cialac-Negolscu A., Pisica G. 1964; The effect of Tween 80 and penicillin on the physiochemical properties of the cell wall in mycobacteria. Studii şi Cercetᾰri de Biochimie. Academie Republicii Populare Romane 7:83–89
    [Google Scholar]
  22. Reese E. T., Maguire A. 1969; Surfactants as stimulants of enzyme production by micro-organisms. Applied Microbiology 17(2):242–245
    [Google Scholar]
  23. Rye R. M., Wiseman D. 1968; Cell size change during growth of Escherichia coli partially inhibited by some antibacterial agents. Journal of Pharmacy and Pharmacology 20: Suppl. 8S
    [Google Scholar]
  24. Vidal-Paruta M. R., King L. D. 1964; Critical micelle concentration of non-ionic surfactants in water and carbon tetrachloride. Journal of Pharmaceutical Sciences 53:1217–1219
    [Google Scholar]
  25. Winsley B. E. 1968 Studies on the effect of polysorbate 80 on the resistance of Pseudomonas aeruginosa Ph.D. Thesis University of Bath:
    [Google Scholar]
  26. Wintermere D. M., Eisenberg W. H., Kirshbaum A. 1957; Turbidimetric assay of tetracycline, chlortetracycline, oxytetracycline, chloramphenicol, neomycin and polymyxin using a single organism. Antibiotics and Chemotherapy 7:190–192
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-68-3-367
Loading
/content/journal/micro/10.1099/00221287-68-3-367
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error