1887

Abstract

SUMMARY: The rate of sphaeroplast formation from yeast was greater with dithiothreitol (DTT) in the reaction mixture than with 2-mercaptoethanol (2-ME) and the sphaeroplast population so produced was more rapidly and completely lysed in a non-isotonic environment. Incubation with DTT, but not 2-ME, allowed rapid formation of sphaeroplasts from yeast in stationary phase. DTT and 2-ME readily released invertase from but only DTT released invertase from DTT-induced release of invertase by both yeasts was dependent on DTT concentration, pH, temperature, metal ions and yeast age, but these yeasts responded differently to oxidation and to release under suboptimal conditions.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-68-3-327
1971-11-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/68/3/mic-68-3-327.html?itemId=/content/journal/micro/10.1099/00221287-68-3-327&mimeType=html&fmt=ahah

References

  1. American Society of Brewing Chemists 1958 Methods of Analysis, 6th edn. pp. 162–168 Wisconsin: American Society of Brewing Chemists Publications;
    [Google Scholar]
  2. Bacon J. S. D., Milne B. D., Taylor I. F., Webley D. M. 1965; Features of the cell wall structure of yeast revealed by the action of enzymes from a non-fruiting Myxobacterium (Cytophaga johnsonii). Biochemical Journal 95:28C
    [Google Scholar]
  3. Davies R., Elvin P. A. 1964; The effect of β-mercaptoethanol on release of invertase and formation of protoplasts of Saccharomyces fragilis. Biochemical Journal 93:8P
    [Google Scholar]
  4. Eddy A. A., Williamson D. H. 1957; A method of isolating protoplasts from yeast. Nature; London: 1791252–1253
    [Google Scholar]
  5. Indge K. J. 1968; The effects of various anions and cations on the lysis of yeast protoplasts by osmotic shock. Journal of General Microbiology 51:425–432
    [Google Scholar]
  6. Kidby D. K., Davies R. 1970a; Thiol-induced release of invertase from cell walls of Saccharomyces fragilis. Biochimica et biophysica acta 201:261–266
    [Google Scholar]
  7. Kidby D. K., Davies R. 1970b; Invertase and disulphide bridges in the yeast wall. Journal of General Microbiology 61:327–333
    [Google Scholar]
  8. Lampen J. O. 1968; External enzymes of yeast; their nature and formation. Antonie van Leeuwenhoek 34:1–18
    [Google Scholar]
  9. Lewis M. J., Phaff H. J. 1965; Effect of environmental conditions on yeast. II. Shock excretion of amino acids. Journal of Bacteriology 87:1389–1396
    [Google Scholar]
  10. Nickerson W. J. 1963; Symposium on biochemical bases of morphogenesis in fungi. IV. Molecular bases of form in yeasts. Bacteriological Reviews 27:305–324
    [Google Scholar]
  11. Phaff H. J. 1963; Cell wall of yeasts. Annual Review of Microbiology 17:15–30
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-68-3-327
Loading
/content/journal/micro/10.1099/00221287-68-3-327
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error